PtPLC, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab Portunus trituberculatus

doi:10.1016/j.fsi.2014.12.017

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	PtPLC, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab Portunus trituberculatus
	Liu, Yuan 1; Cui, Zhaoxia 1,2; Shi, Guohui1,3 ; Luo, Danli 1,3; Wang, Shuangyan 1,3; Wang, Chunlin 4
	2015-03-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	43 期号:1 页码:36-42
文章类型	Article
摘要	Pacifastin-related inhibitor is a new family of serine protease inhibitors that regulate the proteolytic cascade in multiple biological processes. Contrary to the knowledge on the structure and inhibitory mechanism of pacifastin-like members in locust, very little is known about their functions. Here, we report the inhibitory activities in relation to the structural characteristics of pacifastin light chain (PtPLC) gene identified from the swimming crab Portunus trituberculatus. The mature PtPLC and five PLD-related domains with critical residues were expressed in Escherichia coli, and assayed for their activities. The recombinant PtPLC (rPtPLC) displayed inhibitory activities against trypsin and chymotrypsin in a dose dependent manner, with a preference for trypsin. Except for rPtPLC-D4, the other four rPtPLC-related domains could inhibit at least one of serine proteases. The enzyme specificity of PtPLC domains generally corresponded to the nature of the P1 residue at the reactive site. rPtPLC was able to inhibit the growth of Gram-negative bacteria Vibrio alginolyticus and Pseudomonas aeruginosa, but not the Grampositive bacterium and fungus tested. Further phenoloxidase (PO) assay showed the rPtPLC could depress the crab proPO system activation in vitro, and lead to 72.8% inhibition of PO activity at the concentration of 9.11 mu M. It also suppressed proPO activation induced by rPtcSP and rPtSPH1. As the first functional study of the recombinant PLC protein in crustaceans, the present results together indicate that PtPLC functions in the crab immune response possibly via inhibiting bacterial growth and regulating the proPO system. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Portunus Trituberculatus Pacifastin Serine Protease Inhibitor Bacterial Growth Inhibition Prophenoloxidase Activating System
DOI	10.1016/j.fsi.2014.12.017
收录类别	SCI
语种	英语
WOS记录号	WOS:000349731400004
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25381
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuan,Cui, Zhaoxia,Shi, Guohui,et al. PtPLC, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(1):36-42.
APA	Liu, Yuan,Cui, Zhaoxia,Shi, Guohui,Luo, Danli,Wang, Shuangyan,&Wang, Chunlin.(2015).PtPLC, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,43(1),36-42.
MLA	Liu, Yuan,et al."PtPLC, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 43.1(2015):36-42.

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