A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification

doi:10.1016/j.talanta.2014.08.054

	A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification
	Zeng, Yan1,2 ; Wan, Yi 1; Zhang, Dun 1; Qi, Peng1,2
	2015-01-15
发表期刊	TALANTA
卷号	132 页码:59-64
文章类型	Article
摘要	A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease HI (Exo-III) aided cycling amplification has been developed. This magneto-DNA duplex probe contains a partly hybrid fluorophore-modified capture probe and a fluorophore-modified signal probe with magnetic microparticle as carrier. In the presence of a perfectly matched target bacterial DNA, blunt 3'-terminus of the capture probe is formed, activating the Exo-III aided cycling amplification. Thus, Exo-III catalyzes the stepwise removal of mononucleotides from this terminus, releasing both fluorophore-modified signal probe, fluorescent dyes of the capture probe and target DNA. The released target DNA then starts a new cycle, while released fluorescent fragments are recovered with magnetic separation for fluorescence signal collection. This system exhibited sensitive detection of bacterial DNA, with a detection limit of 14 pM because of the unique cleavage function of Exo-III, high fluorescence intensity, and separating function of magneto-DNA duplex probes. Besides this sensitivity, this strategy exhibited excellent selectivity with mismatched bacterial DNA targets and other bacterial species targets and good applicability in real seawater samples, hence, this strategy could be potentially used for qualitative and quantitative analysis of bacteria. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Magneto-dna Duplex Probe Magnetic Microparticle Exonuclease Iii Bacterial Dna Detection
DOI	10.1016/j.talanta.2014.08.054
收录类别	SCI
语种	英语
WOS记录号	WOS:000349278900009
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25364
专题	海洋环境腐蚀与生物污损重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zeng, Yan,Wan, Yi,Zhang, Dun,et al. A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification[J]. TALANTA,2015,132:59-64.
APA	Zeng, Yan,Wan, Yi,Zhang, Dun,&Qi, Peng.(2015).A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification.TALANTA,132,59-64.
MLA	Zeng, Yan,et al."A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification".TALANTA 132(2015):59-64.

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A novel magneto-DNA （2038KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览