| The enhancement mechanism of hydrogen photoproduction in Chlorella protothecoides under nitrogen limitation and sulfur deprivation | |
| Zhang, Litao; He, Meilin; Liu, Jianguo; Liu, JG (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China. | |
| 2014-06-05 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
 |
| 卷号 | 39期号:17页码:8969-8976 |
| 文章类型 | Article |
| 摘要 | The aim of this study was to understand the enhancement mechanism of H-2 photoproduction in Chlorella protothecoides under simultaneous nitrogen limitation and sulfur deprivation (LNS). Nitrogen limitation (LN) rather than sulfur deprivation significantly inhibited relative variable fluorescence at K-step (WK) and J-step (Vi), photochemical efficiency of PSII (photosystem II), F-v/F-m, during the process of incubation in the light. Under such conditions, photosynthetic O-2 evolution decreased and the anaerobiosis was established after 12 h of incubation. The algae generated large amounts of H-2 under nitrogen limitation but generated only trace amounts under sulfur deprivation. Obviously, nitrogen limitation rather than sulfur deprivation was the decisive factor that induced H-2 photoproduction in C. protothecoides under LNS. The LNS culture generated much more H-2 than the LN culture in the presence of DCMU during incubation, suggesting that a PSII-independent electron source contributed many more electrons for transfer to hydrogenase in the LNS culture. PSII electron transport includes linear electron flow (LEF) and cyclic electron flow (CEF) of PSII in C. protothecoides. In the PSII-dependent electron source for H-2 photoproduction, PSII supplies electrons to hydrogenase through the LEF. The LNS culture showed much higher LEF and lower CEF than the LN culture during the H-2 photoproduction phase, as indicated by the large lower quantum yield of PSII electron transport (Phi(PSII)) in the LNS culture in the presence of DCMU. Therefore, compared with nitrogen limitation, simultaneous nitrogen limitation and sulfur deprivation enhanced H-2 photoproduction in C. protothecoides mainly due to enhanced PSII-dependent and -independent electron sources. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. |
| 关键词 | Chlorella Protothecoides Electron Sources H-2 Photoproduction Nitrogen Limitation Sulfur Deprivation |
| 学科领域 | Chemistry ; Electrochemistry ; Energy & Fuels |
| DOI | 10.1016/j.ijhydene.2014.04.045 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336880500007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24246 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Liu, JG (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China. |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Litao,He, Meilin,Liu, Jianguo,et al. The enhancement mechanism of hydrogen photoproduction in Chlorella protothecoides under nitrogen limitation and sulfur deprivation[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2014,39(17):8969-8976. |
| APA | Zhang, Litao,He, Meilin,Liu, Jianguo,&Liu, JG .(2014).The enhancement mechanism of hydrogen photoproduction in Chlorella protothecoides under nitrogen limitation and sulfur deprivation.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,39(17),8969-8976. |
| MLA | Zhang, Litao,et al."The enhancement mechanism of hydrogen photoproduction in Chlorella protothecoides under nitrogen limitation and sulfur deprivation".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 39.17(2014):8969-8976. |
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