| Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila | |
| Liu, Mei1; Wang, Baojie1; Jiang, Keyong1; Gong, Kui1; Sun, Shujuan1; Wang, Lei1; Liu, Wenzhen2; Fu, Yaping2; Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2014-06-01 | |
| 发表期刊 | JOURNAL OF THE WORLD AQUACULTURE SOCIETY
 |
| 卷号 | 45期号:3页码:269-278 |
| 文章类型 | Article |
| 摘要 | Penaeidin is an antimicrobial peptide family found in penaeid shrimp. Penaeidin3 has a broad spectrum of antimicrobial activity and is most effective against certain bacterial species. In this study, the penaeidin3-2 gene was cloned from the Chinese shrimp, Fenneropenaeus chinensis. It was then transformed into rice plants using an Agrobacterium-mediated transformation system. The transformants were verified by polymerase chain reaction (PCR) sequencing and real-time polymerase chain reaction (RT-PCR) analysis. To evaluate the application of penaeidin3-2 expressed by rice plants in the feedstock industry and aquaculture practice, the transgenic rice bran was added to fish feed. The proliferation of microbes in the feed was significantly inhibited by the transgenic rice bran. Furthermore, the mortality of tilapias challenged with Aeromonas hydrophila was also significantly reduced when they were fed with the transgenic bran expressing penaeidin3-2. Their intestinal flora changed when the fish were fed with rice bran expressing the introduced gene for a relatively long period. Fish that survived the challenge had a more intact midgut structure. These results indicate that penaeidin3-2 expressed in rice plants may possess the ability to modulate the intestinal bacterial community and protect the gut structure from damage. |
| 学科领域 | Fisheries |
| DOI | 10.1111/jwas.12121 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340571600003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24241 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China 2.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Mei,Wang, Baojie,Jiang, Keyong,et al. Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2014,45(3):269-278. |
| APA | Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,Gong, Kui.,Sun, Shujuan.,...&Wang, L .(2014).Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,45(3),269-278. |
| MLA | Liu, Mei,et al."Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 45.3(2014):269-278. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Rice Bran Expressing（836KB） | 限制开放 | ODC PDDL | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论