| PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve | |
| Liu, Helu1,2,3; Zhang, Hongkuan1; Zheng, Huaiping1,2; Wang, Shuqi1; Guo, Zhicheng1; Zhang, Guofan4; Zheng, HP (reprint author), Shantou Univ, Key Lab Marine Biotechnol Guangdong Prov, Shantou 515063, Peoples R China. | |
| 2014-12-24 | |
| 发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 卷号 | 62期号:51页码:12384-12391 |
| 文章类型 | Article |
| 摘要 | Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in important physiological processes. However, the endogenous PUFA biosynthesis pathway is poorly understood in marine bivalves. Previously, a fatty acyl desaturase (Fad) with ?5 activity was functionally characterized and an elongase termed Elovl2/5 was reported to efficiently elongate 18:2n-6 and 18:3n-3 to 20:2n-6 and 20:3n-3 respectively in Chlamys nobilis. In this study, another elongase and another Fad were identified. Functional characterization in recombinant yeast showed that the newly cloned elongase can elongate 20:4n-6 and 20:5n-3 to C22 and C24, while the newly cloned scallop Fad exhibited a ?8-desaturation activity, and could desaturate exogenously added PUFA 20:3n-3 and 20:2n-6 to 20:4n-3 and 20:3n-6 respectively, providing the first compelling evidence that noble scallop could de novo biosynthesize 20:5n-3 and 20:4n-6 from PUFA precursors though the "?8 pathway". No Delta 6 or Delta 4 activity was detected for this Fad. Searching against our scallop transcriptome database failed to find any other Fad-like genes, indicating that noble scallop might have limited ability to biosynthesize 22:6n-3. Interestingly, like previously characterized Elovl2/5, the two newly cloned genes showed less efficient activity toward n-3 PUFA substrates than their homologous n-6 substrates, resulting in a relatively low efficiency to biosynthesize n-3 PUFA, implying an adaption to marine environment. |
| 关键词 | Chlamys Nobilis Pufa Biosynthesis Delta 8 Pathway Marine Bivalve |
| 学科领域 | Agriculture ; Chemistry ; Food Science & Technology |
| DOI | 10.1021/jf504648f |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347138500014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24240 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zheng, HP (reprint author), Shantou Univ, Key Lab Marine Biotechnol Guangdong Prov, Shantou 515063, Peoples R China. |
| 作者单位 | 1.Shantou Univ, Key Lab Marine Biotechnol Guangdong Prov, Shantou 515063, Peoples R China 2.Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China 3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Helu,Zhang, Hongkuan,Zheng, Huaiping,et al. PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12384-12391. |
| APA | Liu, Helu.,Zhang, Hongkuan.,Zheng, Huaiping.,Wang, Shuqi.,Guo, Zhicheng.,...&Zheng, HP .(2014).PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12384-12391. |
| MLA | Liu, Helu,et al."PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12384-12391. |
| 条目包含的文件 | ||||||
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| PUFA Biosynthesis Pa（9985KB） | 限制开放 | ODC PDDL | 浏览 | |||
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