| Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments | |
| Qiu, Chuwen1,2; Sun, Jie3; Liu, Mei1; Wang, Baojie1; Jiang, Keyong1; Sun, Shujuan1; Meng, Xiaolin1,2; Luo, Zuoyong1,2; Wang, Lei1; Liu, M (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2014-02-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
 |
| 卷号 | 16期号:1页码:46-53 |
| 文章类型 | Article |
| 摘要 | Peptides derived from shrimp hemocyanin have antimicrobial properties. This is the first report of hemocyanin cDNA (FCHc) cloned from Fenneropenaeus chinensis and recombinant expression of two C-terminal fragments. Based on sequence analysis of Fenneropenaeus chinensis hemocyanin FCHc, we subcloned two FCHc fragments by designing special primers. Two antimicrobial peptides (AMPs) were derived from FCHc (FCHc-C1 and FCHc-C2). The recombinant sequence of FCHc-C1 consisted of 207 bp encoding 69 amino acids and the recombinant sequence of FCHc-C2 consisted of 120 bp encoding 40 amino acids. The results of Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting indicated that recombinant FCHc-C1 and FCHc-C2 peptides (rFCHc-C1 and rFCHc-C2) were expressed successfully. An inhibition assay showed that FCHc-C1 and FCHc-C2 were anionic AMPs with antifungal and antibacterial activities. |
| 关键词 | Fenneropenaeus Chinensis Hemocyanin Antimicrobial Peptides (Amps) Pichia Pastoris Recombinant Expression |
| 学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| DOI | 10.1007/s10126-013-9519-y |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330433700005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24234 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Liu, M (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Qiu, Chuwen,Sun, Jie,Liu, Mei,et al. Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments[J]. MARINE BIOTECHNOLOGY,2014,16(1):46-53. |
| APA | Qiu, Chuwen.,Sun, Jie.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,...&Liu, M .(2014).Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments.MARINE BIOTECHNOLOGY,16(1),46-53. |
| MLA | Qiu, Chuwen,et al."Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments".MARINE BIOTECHNOLOGY 16.1(2014):46-53. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Molecular Cloning of（333KB） | 限制开放 | ODC PDDL | 浏览 | |||
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