| Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum | |
| Ding, Jianfeng1,2; Wang, Rui1,2; Yang, Feng1,2; Zhao, Liqiang1,2; Qin, Yanjie1,2; Zhang, Guofan3; Yan, Xiwu1,2; Yan, XW (reprint author), Dalian Ocean Univ, Dalian 116023, Peoples R China. | |
| 2014-11-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 卷号 | 47期号:1页码:81-89 |
| 文章类型 | Article |
| 摘要 | A novel lysozyme gene (RpLysPh) with high similarity to the bacteriophage lysozymes was identified in Manila clam, Ruditapes philippinarum. The full length cDNA of RpLysPh is 828 bp and contains a 462 bp open reading frame (ORF) that codes for a 154 amino acid protein. Multiple sequence alignment analysis revealed that the three residues essential for catalytic activity in phage-type lysozyme (Glu(20), Asp(29), and Thr(35)) are conserved in RpLysPh. The comparison of the 3D models of RpLysPh and Coxiella burnetii lysozyme also suggested that the active sites involved in the binding of substrate have similar conformations. Phylogenetic analysis suggested that RpLysPh shares a similar origin with the bacterial phage-type lysozyme group. The highest level of expression of RpLysPh was observed in hemocytes, followed by mantle. Induction of RpLysPh expression was observed in gills in response to lipopolysaccharide (LPS), peptidoglycan (PGN), polyinosinic-polycytidylic acid (Poly(I:C)), and whole glucan particles (WGP) challenge. The recombinant protein of RpLysPh showed antibacterial activity against both Gram-positive and Gram-negative bacteria. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Ruditapes Philippinarum Phage-type Like Lysozyme Basal And Temporal Transcriptional Analysis Antibacterial Activity |
| 学科领域 | Immunology ; Zoology |
| DOI | 10.1016/j.dci.2014.06.013 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342275400010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24221 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Yan, XW (reprint author), Dalian Ocean Univ, Dalian 116023, Peoples R China. |
| 作者单位 | 1.Dalian Ocean Univ, Dalian 116023, Peoples R China 2.Engn Res Ctr Shellfish Culture & Breeding Liaonin, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jianfeng,Wang, Rui,Yang, Feng,et al. Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,47(1):81-89. |
| APA | Ding, Jianfeng.,Wang, Rui.,Yang, Feng.,Zhao, Liqiang.,Qin, Yanjie.,...&Yan, XW .(2014).Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,47(1),81-89. |
| MLA | Ding, Jianfeng,et al."Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 47.1(2014):81-89. |
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