| Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve | |
| Liu, Helu1,2; Guo, Zhicheng1,2; Zheng, Huaiping1,2; Wang, Shuqi1,2; Wang, Yajun1,2; Liu, Wenhua1,2; Zhang, Guofan3; Zheng, HP (reprint author), Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China. | |
| 2014-11-01 | |
| 发表期刊 | MOLECULAR BIOLOGY REPORTS
 |
| 卷号 | 41期号:11页码:7437-7445 |
| 文章类型 | Article |
| 摘要 | Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys nobilis cDNA with high homology to vertebrate FADs. Functional characterization in recombinant yeast Saccharomyces cerevisiae showed that scallop FAD exhibited Delta 5-desaturation activity towards both saturated and PUFA substrates. Thus, it efficiently desaturated exogenously added PUFA C20:4(n - 3) and C20:3(n - 6) to C20:5(n - 3) (EPA) and C20:4(n - 6) (ARA) respectively. It also converted the yeast's endogenous C18:0 into C18:1(n - 13), and participated in the biosynthesis of non-methylene-interrupted FA by introducing a double bond to C20:3(n - 3) and C20:2(n - 6) in the Delta 5 carbon. Temporal transcript profile of scallop FAD was studied during early embryonic development. High level of mRNA was found at the beginning of embryogenesis (egg) and noticeable decreases of were observed during larvae development, suggesting maternal FAD mRNA transfer to the embryo. Further, FAD transcripts were detected in all tissues analyzed, with the gonad and hepatopancreas showing the highest expression. |
| 关键词 | Delta 5 Fatty Acid Desaturase Chlamys Nobilis Pufa Biosynthesis Larvae Development Marine Bivalves |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1007/s11033-014-3633-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344101300044 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24214 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zheng, HP (reprint author), Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China. |
| 作者单位 | 1.Shantou Univ, Key Lab Marine Biotechnol Guangdong Prov, Shantou 515063, Peoples R China 2.Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Helu,Guo, Zhicheng,Zheng, Huaiping,et al. Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve[J]. MOLECULAR BIOLOGY REPORTS,2014,41(11):7437-7445. |
| APA | Liu, Helu.,Guo, Zhicheng.,Zheng, Huaiping.,Wang, Shuqi.,Wang, Yajun.,...&Zheng, HP .(2014).Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.MOLECULAR BIOLOGY REPORTS,41(11),7437-7445. |
| MLA | Liu, Helu,et al."Functional characterization of a Delta 5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve".MOLECULAR BIOLOGY REPORTS 41.11(2014):7437-7445. |
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