Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change

doi:10.1371/journal.pone.0090732

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	Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change
	Sun, Yumiao; Li, Fuhua; Sun, Zheng; Zhang, Xiaojun; Li, Shihao; Zhang, Chengsong; Xiang, Jianhai; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
	2014-03-04
发表期刊	PLOS ONE
卷号	9 期号:3 页码:e90732
文章类型	Article
摘要	White spot syndrome virus (WSSV) is the most devastating virosis threatening the shrimp culture industry worldwide. Variations of environmental factors in shrimp culture ponds usually lead to the outbreak of white spot syndrome (WSS). In order to know the molecular mechanisms of WSS outbreak induced by temperature variation and the biological changes of the host at the initial stage of WSSV acute infection, RNA-Seq technology was used to analyze the differentially expressed genes (DEGs) in shrimp with a certain amount of WSSV cultured at 18 degrees C and shrimp whose culture temperature were raised to 25 degrees C. To analyze whether the expression changes of the DEGs were due to temperature rising or WSSV proliferation, the expression of selected DEGs was analyzed by real-time PCR with another shrimp group, namely Group T, as control. Group T didn't suffer WSSV infection but was subjected to temperature rising in parallel. At the initial stage of WSSV acute infection, DEGs related to energy production were up-regulated, whereas most DEGs related to cell cycle and positive regulation of cell death and were down-regulated. Triose phosphate isomerase, enolase and alcohol dehydrogenase involved in glycosis were up-regulated, while pyruvate dehydrogenase, citrate synthase and isocitrate dehydrogenase with NAD as the coenzyme involved in TCA pathway were down-regulated. Also genes involved in host DNA replication, including DNA primase, DNA topoisomerase and DNA polymerase showed down-regulated expression. Several interesting genes including crustin genes, acting binding or inhibiting protein genes, a disintegrin and metalloproteinase domain-containing protein 9 (ADAM9) gene and a GRP 78 gene were also analyzed. Understanding the interactions between hosts and WSSV at the initial stage of acute infection will not only help to get a deep insight into the pathogenesis of WSSV but also provide clues for therapies.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0090732
收录类别	SCI
语种	英语
WOS记录号	WOS:000332475500093
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24188
专题	实验海洋生物学重点实验室
通讯作者	Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Yumiao,Li, Fuhua,Sun, Zheng,et al. Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change[J]. PLOS ONE,2014,9(3):e90732.
APA	Sun, Yumiao.,Li, Fuhua.,Sun, Zheng.,Zhang, Xiaojun.,Li, Shihao.,...&Li, FH .(2014).Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change.PLOS ONE,9(3),e90732.
MLA	Sun, Yumiao,et al."Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change".PLOS ONE 9.3(2014):e90732.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Transcriptome Analys（1305KB）			限制开放	ODC PDDL	浏览