The essential roles of core binding factors CfRunt and CfCBF beta in hemocyte production of scallop Chlamys farreri

doi:10.1016/j.dci.2014.01.008

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	The essential roles of core binding factors CfRunt and CfCBF beta in hemocyte production of scallop Chlamys farreri
	Yue, Feng 1,2; Zhou, Zhi 1; Wang, Lingling 1; Sun, Rui 1,2; Jiang, Qiufen 1,2; Yi, Qilin 1,2; Zhang, Tao1,2 ; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2014-06-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	44 期号:2 页码:291-302
文章类型	Article
摘要	Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBF alpha, subunit and a non-DNA-binding CBF beta subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis. In the present study, two genes encoding Runt (designed as CfRunt) and CBF beta (designed as CfCBF beta) were cloned and characterized from scallop Chlamys farreri. The full-length cDNA of CfRunt and CfCBF beta consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFB domain, respectively. Electrophoretic mobility shift assay demonstrated that the recombinant CfRunt protein (rCfRunt) exhibited solid ability to bind specific DNA, whereas rCfCBF beta could remarkably increase the DNA-binding affinity of rCfRunt. The mRNA transcripts of CfRunt and CfCBF beta could be detected in all tested tissues, especially in hemocytes, heart, hepatopancreas or muscle. After bacterial challenge, the circulating total hemocyte count (THC) of scallop reduced to the lowest level at 6 h (P < 0.05), and then it recovered gradually to the control level at 48-96 h, while the mRNA expressions of CfRunt and CfCBF beta were significant up-regulated between 6 and 48 h (P < 0.05). After CfRunt gene was silenced by RNA interference, the hemocyte renewal rate and circulating THC both decreased significantly (P < 0.05). However, following the RNA interference of CfRunt, the mRNA expression of CfRunt was significantly induced (P < 0.05) and the attenuated hemocyte renewal rate and circulating THC could be repaired partially by LPS stimulation in the CfRunt-silenced scallops. The results collectively indicated that CfRunt and CfCBF beta, as conserved transcription factors, played essential roles in regulating hemocyte production of scallop. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Transcription Factor Runt Cbf Beta Hemocyte Production
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2014.01.008
收录类别	SCI
语种	英语
WOS记录号	WOS:000341069300006
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24175
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yue, Feng,Zhou, Zhi,Wang, Lingling,et al. The essential roles of core binding factors CfRunt and CfCBF beta in hemocyte production of scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,44(2):291-302.
APA	Yue, Feng.,Zhou, Zhi.,Wang, Lingling.,Sun, Rui.,Jiang, Qiufen.,...&Wang, LL .(2014).The essential roles of core binding factors CfRunt and CfCBF beta in hemocyte production of scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,44(2),291-302.
MLA	Yue, Feng,et al."The essential roles of core binding factors CfRunt and CfCBF beta in hemocyte production of scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 44.2(2014):291-302.

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