Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Rock bream (Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection | |
| Zhang, Bao-cun1,2; Zhang, Jian1,2; Xiao, Zhi-zhong1; Sun, Li1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2014-07-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 卷号 | 45期号:1页码:35-42 |
| 文章类型 | Article |
| 摘要 | Viperin in mammals is known to be an antiviral protein that inhibits the replication of diverse DNA and RNA viruses. In teleost, viperin homologues have been identified in a large number of species and, in some cases, are stimulated in transcription by viruses. However, the biological significance of fish viperin protein in antiviral immunity has not been investigated. In this study, we identified a viperin homologue from rock bream (Oplegnathus fasciatus) (named OfVip) and examined its expression pattern, subcellular localization, and immune effect. We found that OfVip expression occurred in eight tissues, and experimental challenge of rock bream with the viral fish pathogen megalocytivirus upregulated OfVip expression in kidney, liver, and spleen. OfVip was localized in the endoplasmic reticulum under normal physiological conditions, and viral infection induced subcellular redistribution of OfVip. Transient transfection of cultured fish cells with an OfVip-expressing plasmid caused enhanced cellular resistance against megalocytivirus challenge. Consistently, in vivo study showed that rock bream overexpressing OfVip exhibited significantly reduced viral loads in tissues following experimental infection with megalocytivirus. Furthermore, OfVip upregulated the expression of a wide range of immune genes, including those that are known to participate in antiviral immunity. Taken together, these results indicate for the first time that a teleost viperin is a virus-responsive protein that is modulated in subcellular localization by viral infection-, and that viperin regulates the immune reactions of the host fish in a manner that augments resistance against viral infection. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Oplegnathus Fasciatus Viperin Antiviral Immunoregulation |
| 学科领域 | Immunology ; Zoology |
| DOI | 10.1016/j.dci.2014.02.001 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335624800004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24160 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Bao-cun,Zhang, Jian,Xiao, Zhi-zhong,et al. Rock bream (Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,45(1):35-42. |
| APA | Zhang, Bao-cun,Zhang, Jian,Xiao, Zhi-zhong,Sun, Li,&Sun, L .(2014).Rock bream (Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,45(1),35-42. |
| MLA | Zhang, Bao-cun,et al."Rock bream (Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 45.1(2014):35-42. |
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