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| NKLP27: A Teleost NK-Lysin Peptide that Modulates Immune Response, Induces Degradation of Bacterial DNA, and Inhibits Bacterial and Viral Infection | |
Zhang, Min1,2; Li, Mo-fei1,4 ; Sun, Li1,3; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China.
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| 2014-09-02 | |
| 发表期刊 | PLOS ONE
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| 卷号 | 9期号:9页码:e106543 |
| 文章类型 | Article |
| 摘要 | NK-lysin is an antimicrobial protein produced by cytotoxic T lymphocytes and natural killer cells. In this study, we examined the biological property of a peptide, NKLP27, derived from tongue sole (Cynoglossus semilaevis) NK-lysin. NKLP27 is composed of 27 amino acids and shares little sequence identity with known NK-lysin peptides. NKLP27 possesses bactericidal activity against both Gram-negative and Gram-positive bacteria including common aquaculture pathogens. The bactericidal activity of NKLP27 was dependent on the C-terminal five residues, deletion of which dramatically reduced the activity of NKLP27. During its interaction with the target bacterial cells, NKLP27 destroyed cell membrane integrity, penetrated into the cytoplasm, and induced degradation of genomic DNA. In vivo study showed that administration of tongue sole with NKLP27 before bacterial and viral infection significantly reduced pathogen dissemination and replication in tissues. Further study revealed that fish administered with NKLP27 exhibited significantly upregulated expression of the immune genes including those that are known to be involved in antibacterial and antiviral defense. These results indicate that NKLP27 is a novel antimicrobial against bacterial and viral pathogens, and that the observed effect of NKLP27 on bacterial DNA and host gene expression adds new insights to the action mechanism of fish antimicrobial peptides. |
| 学科领域 | Science & Technology - Other Topics |
| DOI | 10.1371/journal.pone.0106543 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341231500112 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24134 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Peoples R China 3.Zhejiang Univ, Collaborat Innovat Ctr Deep Sea Biol, Hangzhou 310003, Zhejiang, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Li, Mo-fei,Sun, Li,et al. NKLP27: A Teleost NK-Lysin Peptide that Modulates Immune Response, Induces Degradation of Bacterial DNA, and Inhibits Bacterial and Viral Infection[J]. PLOS ONE,2014,9(9):e106543. |
| APA | Zhang, Min,Li, Mo-fei,Sun, Li,&Sun, L .(2014).NKLP27: A Teleost NK-Lysin Peptide that Modulates Immune Response, Induces Degradation of Bacterial DNA, and Inhibits Bacterial and Viral Infection.PLOS ONE,9(9),e106543. |
| MLA | Zhang, Min,et al."NKLP27: A Teleost NK-Lysin Peptide that Modulates Immune Response, Induces Degradation of Bacterial DNA, and Inhibits Bacterial and Viral Infection".PLOS ONE 9.9(2014):e106543. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| NKLP27 A Teleost NK-（1127KB） | 限制开放 | ODC PDDL | 浏览 | |||
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