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| Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity | |
Zhang, Qingli1; Huang, Jie1; Li, Fuhua2; Liu, Shuang1; Liu, Qinghui1; Wei, Jiankai2 ; Liang, Gaofeng1; Xiang, Jianhai2; Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2014-03-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 37期号:1页码:38-45 |
| 文章类型 | Article |
| 摘要 | Peroxiredoxins (Prx) are a family of antioxidant proteins and perform important functions in intracellular signal transduction. Here, we report a Prx gene from Chinese shrimp Fenneropenaeus chinensis. The full-length cDNA of FcPrx gene contained an open reading frame of 735 bp encoding a polypeptide of 275 amino acids. The molecular mass of the deduced amino acid of FcPrx is 27445.43 Da with an estimated pI of 5.71. Sequence comparison showed that the FcPrx shares high identities with Prx IVs and it was named FcPrx4. A real-Time PCR (qRT-PCR) assay was developed to assess the mRNA expression of FcPrx4 in different tissues and temporal expression in hemocytes and hepatopancreas of E chinensis challenged by white spot syndrome virus (WSSV). Transcripts of FcPrx4 can be detected in all tissues examined. The expression of FcPrx4 showed significant up-regulation in shrimp hemocytes and hepatopancreas after artificial infection with WSSV. A fusion protein containing FcPrx4 was produced in vitro and was confirmed by Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) assay. And activity analysis indicated that the recombinant FcPrx4 proteins can reduce H2O2 in the presence of dithiothreitol. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Peroxiredoxin (Prx) White Spot Syndrome Virus (Wssv) Infection Recombinant Protein Antioxidant Activity |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.12.026 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000333717100005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24129 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qingli,Huang, Jie,Li, Fuhua,et al. Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,37(1):38-45. |
| APA | Zhang, Qingli.,Huang, Jie.,Li, Fuhua.,Liu, Shuang.,Liu, Qinghui.,...&Xiang, JH .(2014).Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity.FISH & SHELLFISH IMMUNOLOGY,37(1),38-45. |
| MLA | Zhang, Qingli,et al."Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity".FISH & SHELLFISH IMMUNOLOGY 37.1(2014):38-45. |
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