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| Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production | |
| Gao, Yongqiang1,2; Liu, Changshui1,2; Ding, Yamei3; Sun, Chao1; Zhang, Rubing1; Xian, Mo1,4; Zhao, Guang1,4; Xian, M (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China. | |
| 2014-05-16 | |
| 发表期刊 | PLOS ONE
 |
| 卷号 | 9期号:5页码:e97845 |
| 文章类型 | Article |
| 摘要 | Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In our previous study, a pathway for P3HP production was constructed in recombinant Esecherichia coli. Seven exogenous genes in P3HP synthesis pathway were carried by two plasmid vectors. However, the P3HP production was severely suppressed by strain instability due to plasmid loss. In this paper, two strategies, chromosomal gene integration and plasmid addiction system (PAS) based on amino acid anabolism, were applied to construct a genetically stable strain. Finally, a combination of those two methods resulted in the best results. The resultant strain carried a portion of P3HP synthesis genes on chromosome and the others on plasmid, and also brought a tyrosine-auxotrophy based PAS. In aerobic fed-batch fermentation, this strain produced 25.7 g/L P3HP from glycerol, about 2.5-time higher than the previous strain with two plasmids. To the best of our knowledge, this is the highest P3HP production from inexpensive carbon sources. |
| 学科领域 | Science & Technology - Other Topics |
| DOI | 10.1371/journal.pone.0097845 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339614800076 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24102 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xian, M (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yongqiang,Liu, Changshui,Ding, Yamei,et al. Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production[J]. PLOS ONE,2014,9(5):e97845. |
| APA | Gao, Yongqiang.,Liu, Changshui.,Ding, Yamei.,Sun, Chao.,Zhang, Rubing.,...&Xian, M .(2014).Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production.PLOS ONE,9(5),e97845. |
| MLA | Gao, Yongqiang,et al."Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production".PLOS ONE 9.5(2014):e97845. |
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