Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515

doi:10.1007/s10811-013-0223-0

IOCAS-IR > 实验海洋生物学重点实验室

	Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515
	Wu, Songcui1,2 ; Zhang, Baoyu 1; Huang, Aiyou 1; Huan, Li1,2 ; He, Linwen 1; Lin, Apeng 1; Niu, Jianfeng 1; Wang, Guangce 1; Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China.
	2014-08-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
卷号	26 期号:4 页码:1659-1668
文章类型	Article
摘要	Diatoms and dinoflagellates not only have extensive distribution and a huge biomass in marine ecosystems, but also have high lipid accumulation in nature or after physiological and genetic modification, which indicates that these organisms may be optimal candidate algal strains for biodiesel production. In this study, we determined the content of intracellular neutral lipids (triacylglycerol [TAG]) in the dinoflagellate Prorocentrum micans and in the diatom Phaeodactylum tricornutum using NR and BODIPY 505/515 staining. The freshwater green alga Scenedesmus obliquus was used as a control. Optimum concentrations of 1.000 and 1.500 mu g mL(-1) were determined for neutral lipid Nile red (NR) staining in P. micans and P. tricornutum. Unlike NR staining, the optimal concentrations of BODIPY 505/515 staining in P. micans and P. tricornutum were lower, at 0.100 and 0.075 mu g mL(-1), respectively. High correlation coefficients of R (2) = 0.990 and R (2) = 0.989 were obtained for P. micans and P. tricornutum intracellular neutral lipid content and the relative fluorescence intensity with NR staining, while the reference alga, S. obliquus, had a relatively low correlation coefficient of R (2) = 0.908 when stained with NR. The neutral lipid content determined by thin-layer chromatography-flame ionization detector matched the analytical data from fluorescence measurements. These results indicated that NR and BODIPY 505/515 staining can be used as an excellent high-throughput approach to screen marine diatoms and dinoflagellates.
关键词	Marine Microalga Bodipy 505/515 Nile Red Neutral Lipid Triacylglycerol Biodiesel
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-013-0223-0
收录类别	SCI
语种	英语
WOS记录号	WOS:000340101100005
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24101
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wu, Songcui,Zhang, Baoyu,Huang, Aiyou,et al. Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(4):1659-1668.
APA	Wu, Songcui.,Zhang, Baoyu.,Huang, Aiyou.,Huan, Li.,He, Linwen.,...&Wang, GC .(2014).Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515.JOURNAL OF APPLIED PHYCOLOGY,26(4),1659-1668.
MLA	Wu, Songcui,et al."Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515".JOURNAL OF APPLIED PHYCOLOGY 26.4(2014):1659-1668.

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