Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus | |
Jiang, Hao1,2; Li, Fuhua1; Zhang, Jiquan; Zhang, Jinkang2; Huang, Bingxin1; Yu, Yang1,2 ; Xiang, Jianhai1; Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
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| 2014-02-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
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| 卷号 | 16期号:1页码:111-123 |
| 文章类型 | Article |
| 摘要 | Fenneropenaeus chinensis (Chinese shrimp) culture industry, like other Penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. To better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. Firstly, the soluble hepatopancreas protein samples in adult Chinese shrimp among control, heat-inactivated Vibrio-challenged and white spot syndrome virus-infected groups were separated by 2-DE (pH range, 4-7; sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and pH range, 3-10; tricine-SDS-PAGE). Then the differentially expressed protein spots (a parts per thousand yen1.5-fold or a parts per thousand currency sign0.67-fold averagely of controls) were analyzed by LC-ESI-MS/MS. Using Mascot online database searching algorithm and SEQUEST searching program, 48 and 49 differentially expressed protein spots were successfully identified in response to Vibrio and white spot syndrome virus infection, respectively. Based on these results, we discussed the mechanism of immune response of the shrimp and shed light on the differences between immune response of shrimp toward Vibrio and white spot syndrome virus. This study also set a basis for further analyses of some key genes in immune response of Chinese shrimp. |
| 关键词 | Vibrio Wssv Shrimp Proteomics 2-de |
| 学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| DOI | 10.1007/s10126-013-9538-8 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330433700011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24094 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Jiang, Hao,Li, Fuhua,Zhang, Jiquan,et al. Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus[J]. MARINE BIOTECHNOLOGY,2014,16(1):111-123. |
| APA | Jiang, Hao.,Li, Fuhua.,Zhang, Jiquan.,Zhang, Jinkang.,Huang, Bingxin.,...&Xiang, JH .(2014).Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus.MARINE BIOTECHNOLOGY,16(1),111-123. |
| MLA | Jiang, Hao,et al."Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus".MARINE BIOTECHNOLOGY 16.1(2014):111-123. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Comparison of Protei（445KB） | 限制开放 | ODC PDDL | 浏览 | |||
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