Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis

doi:10.1080/07924259.2014.905501

IOCAS-IR > 实验海洋生物学重点实验室

	Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis
	Wen, Rong 1,2; Li, Fuhua 1; Xie, Yusu 1; Li, Shihao 1; Zhang, Chengsong1 ; Yu, Kuijie 1; Xiang, Jianhai1 ; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-09-01
发表期刊	INVERTEBRATE REPRODUCTION & DEVELOPMENT
卷号	58 期号:3 页码:226-234
文章类型	Article
摘要	The spermatogonial stem-cell renewal factor (SSRF) was named since its function in spermatogonial mitosis was reported in Japanese eel. Our previous study showed that a homolog of SSRF was highly expressed in the ovary of triploid shrimp, but not expressed in the ovary of diploid shrimp. To understand the function of SSRF in shrimp, the full-length cDNA of ssrf gene was cloned from Chinese shrimp Fenneropenaeus chinensis (Fcssrf) and its expression was analyzed. The full length of Fcssrf cDNA was 2588 bp and it contained an open reading frame encoding 450 amino acids. The predicted tertiary structure of FcSSRF was very similar to that of SSRF/eSRS34 from Anguilla japonica and TP/PD-ECGF from Homo sapiens. RT-PCR analysis showed that the Fcssrf was highly expressed in nerve, testis, hepatopancreas, gill, and stomach rather than in ovary. Expression of Fcssrf mRNA was not detected during embryonic stages and larval stages, from the nauplii to the post-larvae stage, in diploid, and triploid shrimp. However, it began to be expressed in juvenile stages (June-September) in diploid and triploid shrimp. Immunohistochemical analyses showed that FcSSRF was identified in both the diploid testis and triploid ovary. We inferred that the Fcssrf might be related to testis development.
关键词	Ovary Testis Development Triploid Penaeid Shrimp
学科领域	Reproductive Biology ; Zoology
DOI	10.1080/07924259.2014.905501
收录类别	SCI
语种	英语
WOS记录号	WOS:000338105700009
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24088
专题	实验海洋生物学重点实验室
通讯作者	Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wen, Rong,Li, Fuhua,Xie, Yusu,et al. Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT,2014,58(3):226-234.
APA	Wen, Rong.,Li, Fuhua.,Xie, Yusu.,Li, Shihao.,Zhang, Chengsong.,...&Li, FH .(2014).Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis.INVERTEBRATE REPRODUCTION & DEVELOPMENT,58(3),226-234.
MLA	Wen, Rong,et al."Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis".INVERTEBRATE REPRODUCTION & DEVELOPMENT 58.3(2014):226-234.

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