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| An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation | |
Sun, Lingling1,2; Huan, Pin1 ; Wang, Hongxia1; Liu, Fengsong2; Liu, Baozhong1; Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2014-05-01 | |
| 发表期刊 | MOLECULAR BIOLOGY REPORTS
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| 卷号 | 41期号:5页码:2757-2765 |
| 文章类型 | Article |
| 摘要 | The epidermal growth factor receptor (EGFR) is an important receptor tyrosine kinase member in animals, which plays versatile functions in development, growth, tissue regeneration etc. Current knowledge on EGFR is poor in bivalve mollusks. In this study, we cloned and analyzed an EGFR gene from the Pacific oyster Crassostrea gigas (cgegfr). A 5,731 bp full-length cDNA of cgegfr was obtained, encoding a peptide with 1,494 amino acids which exhibited a typical EGFR structure, including an extracellular region, a single transmembrane region and an intracellular region. A conserved tyrosine kinase domain was predicted in the intracellular region, while the extracellular region responsible for ligand binding showed comparatively poor conservation. Expression analysis revealed that cgefgr was expressed widely in C. gigas tissues and a highest expression level was observed in adductor tissue. Expression of cgegfr was revealed to be up-regulated during wound healing of mantle, indicating that EGFR might function in the cell proliferation and migration during wound healing. Further functional analysis of cgegfr was conducted in mouse myoblast cell line C2C12, in which different parts of cgegfr were expressed and their effects were measured. The results revealed that cgegfr was able to accelerate cell proliferation of C2C12 cells and the transmembrane region was necessary for self-activation of truncated cgegfr. Our results would provide supports for further studies on the roles of cgegfr in development and growth in C. gigas. |
| 关键词 | Receptor Tyrosine Kinase Cell Proliferation Tissue Regeneration Bivalve |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1007/s11033-014-3130-9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335734400007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24069 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Hebei Univ, Shijiazhuang, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lingling,Huan, Pin,Wang, Hongxia,et al. An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation[J]. MOLECULAR BIOLOGY REPORTS,2014,41(5):2757-2765. |
| APA | Sun, Lingling,Huan, Pin,Wang, Hongxia,Liu, Fengsong,Liu, Baozhong,&Liu, BZ .(2014).An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation.MOLECULAR BIOLOGY REPORTS,41(5),2757-2765. |
| MLA | Sun, Lingling,et al."An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation".MOLECULAR BIOLOGY REPORTS 41.5(2014):2757-2765. |
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