A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)

doi:10.1007/s11033-013-2888-5

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	A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)
	Deng, Yunyan 1; Wang, Xiuliang 1; Guo, Hui 1,2; Duan, Delin 1; Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014
发表期刊	MOLECULAR BIOLOGY REPORTS
卷号	41 期号:1 页码:529-536
文章类型	Article
摘要	The full-length cDNA sequence of a trehalose-6-phosphate synthase gene from Saccharina japonica (designated as SjaTPS) (Accession: KC578568) was isolated based on homologous cloning and RACE-PCR. It was 4,127 bp, with 320 bp 5'-UTR, 21 bp 3'-UTR, and open reading frame (ORF) of 3,786 bp. The deduced 1,261 amino acids characterized with predicted molecular weight of 137.84 kDa and theoretical isoelectric point of 7.12. The SjaTPS had one N-terminal CBM20 (family 20 carbohydrate-binding module) domain, one TPS domain (trehalose-6-phosphate synthase) in the middle region and a single TPP (trehalose-6-phosphate phosphatase) domain near the C-terminus. Structural analysis suggested that the SjaTPS putatively functioned as trehalose-6-phosphate synthase, and might be related to laminaran metabolism in S. japonica. Homology analysis indicated that the SjaTPS shared 49-70 % similarities with the 13 known TPS sequences of other algae; only 55 % amino acid similarities were detected between SjaTPS and the previously reported TPS sequence of S. japonica (Accession: DQ666325). Phylogenetic analysis revealed close affinity between SjaTPS and TPS of brown alga Ectocarpus siliculosus (Accession: CBJ29609). Transcriptional analysis showed that desiccation greatly enhanced SjaTPS expression and the maximum appeared at 3 h, which was about 300-fold compared to that of the start, implied that SjaTPS was involved with drought adaption in kelp. In vitro expression of SjaTPS showed that one distinct band existed at similar to 115 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results increased the knowledge of trehalose-6-phosphate synthase properties in S. japonica and also important for better understanding the role trehalose plays in kelp abiotic tolerance for adaption to the sublittoral habitats.
关键词	Cbm20 Domain Drought Adaption Saccharina Japonica Trehalose-6-phosphate Synthase Tps Domain Tpp Domain
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-013-2888-5
收录类别	SCI
语种	英语
WOS记录号	WOS:000329100500057
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24064
专题	实验海洋生物学重点实验室
通讯作者	Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Deng, Yunyan,Wang, Xiuliang,Guo, Hui,et al. A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)[J]. MOLECULAR BIOLOGY REPORTS,2014,41(1):529-536.
APA	Deng, Yunyan,Wang, Xiuliang,Guo, Hui,Duan, Delin,&Duan, DL .(2014).A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae).MOLECULAR BIOLOGY REPORTS,41(1),529-536.
MLA	Deng, Yunyan,et al."A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)".MOLECULAR BIOLOGY REPORTS 41.1(2014):529-536.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
A trehalose-6-phosph（430KB）			限制开放	ODC PDDL	浏览