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| A new fibrinogen-related protein from Argopecten irradians (AiFREP-2) with broad recognition spectrum and bacteria agglutination activity | |
Yang, Chuanyan1; Wang, Leilei1,2; Zhang, Huan1; Wang, Lingling1; Huang, Mengmeng1,2 ; Sun, Zhibin1; Sun, Ying1,2; Song, Linsheng1; Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2014-05-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 38期号:1页码:221-229 |
| 文章类型 | Article |
| 摘要 | Fibrinogen-related proteins (FREPs) are a kind of pattern recognition receptors (PRRs) containing fibrinogen-like (FBG) domains, and they play curial roles in the innate immune response. In the present study, a new FREP protein was identified from bay scallop Argopecten irradians (designated as AiFREP-2). The full-length cDNA of AiFREP-2 was of 1299 bp with an open reading frame of 762 bp encoding a polypeptide of 253 amino acids, including a signal sequence and an FBG domain. The FBG domain in AiFREP-2 was highly similar to those of ficolins, tenascins and other FREPs. The mRNA expression of AiFREP-2 could be detected in all the examined tissues with the highest level in gill. The mRNA expression of AiFREP-2 in hemocytes was significantly up-regulated post the stimulation of lipopolysaccharide (LP5), peptidoglycan (PGN) and beta-glucan (GLU) (P < 0.01). The recombinant AiFREP-2 (rAiFREP-2) could bind not only different PAMP ligands including LPS, PGN and GLU, but also various microbes including Gram-negative bacteria (Vibrio anguillarum), Gram-positive bacteria (Staphylococcus aureus) and fungus (Pichia pastoris and Yarrowia lipolytica). Additionally, rAiFREP-2 exhibited obvious agglutination activity towards Gram-negative bacteria V. anguillarum and Gram positive bacteria S. aureus. The results indicated that AiFREP-2 was involved in the immune response against Gramnegative bacteria, Gram-positive bacteria and fungus as a PRR in bay scallop, and the information was helpful to understand the innate immune defense mechanisms of mollusks. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Argopecten Irradians Frep Innate Immunity Pattern Recognition Receptor Agglutination Activity |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2014.03.025 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336353700029 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24062 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Chuanyan,Wang, Leilei,Zhang, Huan,et al. A new fibrinogen-related protein from Argopecten irradians (AiFREP-2) with broad recognition spectrum and bacteria agglutination activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):221-229. |
| APA | Yang, Chuanyan.,Wang, Leilei.,Zhang, Huan.,Wang, Lingling.,Huang, Mengmeng.,...&Song, LS .(2014).A new fibrinogen-related protein from Argopecten irradians (AiFREP-2) with broad recognition spectrum and bacteria agglutination activity.FISH & SHELLFISH IMMUNOLOGY,38(1),221-229. |
| MLA | Yang, Chuanyan,et al."A new fibrinogen-related protein from Argopecten irradians (AiFREP-2) with broad recognition spectrum and bacteria agglutination activity".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):221-229. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A new fibrinogen-rel（2438KB） | 限制开放 | ODC PDDL | 浏览 | |||
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