A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions

doi:10.1016/j.ijbiomac.2014.06.020

IOCAS-IR > 实验海洋生物学重点实验室

	A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions
	Zhang, Jiquan 1; Wang, Jing 1,2; Gui, Tianshu1,2 ; Sun, Zheng 1; Xiang, Jianhai1 ; Zhang, JQ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-09-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号	70 页码:246-250
文章类型	Article
摘要	A full-length copper-induced metallothionein (EcMT-Cu) cDNA was obtained from Exopalaemon carinicauda (Holthuis) and it contained a 198bp open reading frame that encoded a peptide with 65 amino acid residues. Twenty-one cysteines were found in deduced amino acid sequence and the cysteine (Cys)-rich characteristic was also reported in different types of metallothioneins from other species. EcMT-Cu mRNA expression profile showed that it is the hepatopancreas specific gene. The expression of EcMT-Cu was extremely different when shrimp were exposed to seawater containing 50 mu M CuSO4 or 2.5 mu M CdCl2. The expression of EcMT-Cu in shrimp was significantly up-regulated at 12 and 24h after exposure to CuSO4, however, its expression was not induced compared to that of pretreatment (p > 0.05) when shrimp were exposed to CdCl2. The transcript of EcMT-Cu was found to be extremely low at gastrula and nauplius stage and expression of EcMT-Cu could be detected from egg protozoa stage. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Metallothionein E. Carinicauda Developmental Stage
学科领域	Biochemistry & Molecular Biology
DOI	10.1016/j.ijbiomac.2014.06.020
收录类别	SCI
语种	英语
WOS记录号	WOS:000342478800035
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24057
专题	实验海洋生物学重点实验室海洋生物技术研发中心
通讯作者	Zhang, JQ (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Jiquan,Wang, Jing,Gui, Tianshu,et al. A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,70:246-250.
APA	Zhang, Jiquan,Wang, Jing,Gui, Tianshu,Sun, Zheng,Xiang, Jianhai,&Zhang, JQ .(2014).A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,70,246-250.
MLA	Zhang, Jiquan,et al."A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 70(2014):246-250.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
A copper-induced met（851KB）			限制开放	ODC PDDL	浏览