A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri

doi:10.1016/j.fsi.2014.05.010

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	A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri
	Yue, Feng 1,2; Zhou, Zhi 1; Wang, Lingling 1; Wang, Mengqiang1 ; Song, Linsheng 1; Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China.
	2014-08-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	39 期号:2 页码:125-135
文章类型	Article
摘要	GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence "GATA", and involved in a myriad of cellular processes. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. The full-length cDNA of CfGATA is of 2058 bp encoding a predicted polypeptide of 457 amino acids with two conserved zinc finger domains, which shared high similarity with other reported GATA1/2/3 proteins. The mRNA transcripts of CfGATA showed higher expression in gills, hepatopancreas, hemocytes and heart, and the CfGATA protein expressed in HEK293 cells was found to be localized specifically in the nuclei. The recombinant CfGATA protein (rCfGATA) exhibited strong ability to bind specific WGATAR DNA sequence by electrophoretic mobility shift assay in vitro. After CfGATA gene was silenced by RNA interference, the hemocyte renewal rate and circulating total hemocyte count (THC) decreased significantly, which was 7.85-fold and 19.46-fold lower than that of PBS control, respectively (P < 0.05). After LPS stimulation, the expression level of CfGATA mRNA decreased significantly in the hemocytes of PBS or EGFP dsRNA treated scallops, which was accompanied by the increase of hemocyte renewal rate and the reduced circulating THC at 24 h. In contrast, the hemocyte renewal rate and circulating THC did not change significantly in CfGATA gene interfered scallops after LPS stimulation. These results suggested that CfGATA, as a conserved GATA1/2/3 transcription factor, plays essential roles in regulating hemocyte production of scallop. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Transcription Factor Gata Hemocyte Production Hematopoiesis
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2014.05.010
收录类别	SCI
语种	英语
WOS记录号	WOS:000339773000001
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24056
专题	实验海洋生物学重点实验室
通讯作者	Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yue, Feng,Zhou, Zhi,Wang, Lingling,et al. A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):125-135.
APA	Yue, Feng,Zhou, Zhi,Wang, Lingling,Wang, Mengqiang,Song, Linsheng,&Song, LS .(2014).A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,39(2),125-135.
MLA	Yue, Feng,et al."A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):125-135.

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