Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino | |
Ma Deyou1,2; Yang Hongsheng1 ; Sun Lina1,2
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| 2014-12-01 | |
| 发表期刊 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
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| ISSN | 1672-5182 |
| 卷号 | 13期号:6页码:1033-1042 |
| 摘要 | Sea cucumber (Apostichopus japonicus) is one of the most important aquaculture animals in China. Usually its normal body color is black that fits its living environment. The juvenile individuals obtained by crossing albino sea cucumber segregated in body color. To document the transcriptome difference between albino associating sea cucumber and the control, we sequenced their transcriptomes with RNA-seq. Approximately, 4.790 million (M) and 4.884 M reads, 200 nt in length, were generated from the body wall of albino associating sea cucumber and the control, respectively, from them, 9550 (46.81%) putative genes were identified. In total, 583 genes were found to express differentially between albino associating sea cucumber and the control. Of these differentially expressed genes (DEGs), 4.8% changed more than five-folds. The expression levels of eight DEGs were confirmed with real-time PCR. The changing trend of these DEGs detected with real-time PCR agreed well with that detected with RNA-seq, although the change degree of some DEGs was different. Four significantly enriched pathways were identified for DEGs, which included phagocytosis, Staphylococcus aureus infection, ECM-receptor interaction and focal adhesion. These pathways were helpful for understanding the physiological difference between albino associating sea cucumber and the control. |
| 关键词 | Apostichopus japonicus sezparation gene expression profiling RNA-seq real-time PCR |
| DOI | 10.1007/s11802-014-2297-y |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[40976089]; National Key Technology Support Program of China[2011BAD13B02]; National Oceanic Public Welfare Industry Special Scientific Research of China[201205023]; Chinese National 863 Project[2012AA10A412] |
| WOS研究方向 | Oceanography |
| WOS类目 | Oceanography |
| WOS记录号 | WOS:000344788700020 |
| 出版者 | OCEAN UNIV CHINA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24036 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang Hongsheng |
| 作者单位 | 1.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Ma Deyou,Yang Hongsheng,Sun Lina. Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(6):1033-1042. |
| APA | Ma Deyou,Yang Hongsheng,&Sun Lina.(2014).Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino.JOURNAL OF OCEAN UNIVERSITY OF CHINA,13(6),1033-1042. |
| MLA | Ma Deyou,et al."Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino".JOURNAL OF OCEAN UNIVERSITY OF CHINA 13.6(2014):1033-1042. |
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