Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal | |
| Zhang, Wei Wei1; Andong, Zheng Yang2; Zhang, Min3; Wang, Qiao Ning1; Wei, Ying Qin4; Chen, Ling Xin1; Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. | |
| 2014-09-01 | |
| 发表期刊 | ANNALS OF MICROBIOLOGY
 |
| 卷号 | 64期号:3页码:1231-1238 |
| 文章类型 | Article |
| 摘要 | Ammonia (referring to both NH3 and NH4 (+)), is one of the most toxic nitrogen forms, and is toxic to many aquatic organisms. The present study identified a heterotrophic nitrifier Proteus mirabilis strain V7, isolated from the coastal seawater. Almost 100 % of 82 mg L-1 NH4 (+)-N was removed within 48 h, while 51.2 % of total inorganic nitrogen was removed within 96 h after inoculation of Proteus mirabilis strain V7. Moreover, Proteus mirabilis strain V7 could remove NH4 (+)-N in a seawater matrix, but with much lower efficiency. Three water samples containing NH4 (+)-N at concentrations of 64.40, 18.00 and 9.82 mg L-1 were chosen to further test the NH4 (+)-N removal ability of Proteus mirabilis strain V7. All of the NH4 (+)-N was removed, with only a trace production of NO2 (-) and NO3 (-). A putative subunit of ammonia monooxygenase from Proteus mirabilis strain V7, amoA (Pm), was cloned and its mRNA level in the presence of NH4 (+) was characterized by real time reverse transcriptase PCR (RT-PCR). A 1.8-fold increase in the mRNA level of amoA (Pm) was observed, which indicated that AmoA may be involved in NH4 (+) oxidization. Our results indicated that Proteus mirabilis strain V7 could potentially be used as new resource for the treatment of NH4 (+)-N contaminated environments. |
| 关键词 | Ammonia Proteus Mirabilis nh4+-n Removal Ammonia Monooxygenase Mrna Level |
| 学科领域 | Biotechnology & Applied Microbiology ; Microbiology |
| DOI | 10.1007/s13213-013-0764-0 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340087200037 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24022 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 4.Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei Wei,Andong, Zheng Yang,Zhang, Min,et al. Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal[J]. ANNALS OF MICROBIOLOGY,2014,64(3):1231-1238. |
| APA | Zhang, Wei Wei.,Andong, Zheng Yang.,Zhang, Min.,Wang, Qiao Ning.,Wei, Ying Qin.,...&Chen, LX .(2014).Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal.ANNALS OF MICROBIOLOGY,64(3),1231-1238. |
| MLA | Zhang, Wei Wei,et al."Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal".ANNALS OF MICROBIOLOGY 64.3(2014):1231-1238. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Isolation and charac（1042KB） | 限制开放 | ODC PDDL | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论