Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria

doi:10.1016/j.bios.2013.11.080

	Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria
	Wan, Yi 1; Sun, Yan 1; Qi, Peng1,2 ; Wang, Peng1 ; Zhang, Dun 1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2014-05-15
发表期刊	BIOSENSORS & BIOELECTRONICS
卷号	55 页码:289-293
文章类型	Article
摘要	Nanomaterial-based 'chemical nose' sensor with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. A sensor array containing three quaternized magnetic nanoparticles (q-MNPs)-fluorescent polymer systems has been designed to identify and quantify bacteria. The bacterial cell membranes disrupt the q-MNP-fluorescent polymer, generating unique fluorescence response array. The response intensity of the array is dependent on the level of displacement determined by the relative q-MNP-fluorescent polymer binding strength and bacteria cells-MNP interaction. These characteristic responses show a highly repeatable bacteria cells and can be differentiated by linear discriminant analysis (LDA). Based on the array response matrix from LDA, our approach has been used to measure bacteria with an accuracy of 87.5% for 107 cfu mL-1 within 20 min. Combined with UV-vis measurement, the method can be successfully performed to identify and detect eight different pathogen samples with an accuracy of 96.8%. The measurement system has a potential for further applications and provides a facile and simple method for the rapid analysis of protein, DNA, and pathogens. (C) 2013 Elsevier B.V. All rights reserved.
关键词	Quatemized Magnetic Nanoparticles Bacteria Detection Fluorescent Polymer Linear Discriminant Analysis
学科领域	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
DOI	10.1016/j.bios.2013.11.080
收录类别	SCI
语种	英语
WOS记录号	WOS:000332426100046
引用统计	被引频次：56[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/23885
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wan, Yi,Sun, Yan,Qi, Peng,et al. Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria[J]. BIOSENSORS & BIOELECTRONICS,2014,55:289-293.
APA	Wan, Yi,Sun, Yan,Qi, Peng,Wang, Peng,Zhang, Dun,&Zhang, D .(2014).Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria.BIOSENSORS & BIOELECTRONICS,55,289-293.
MLA	Wan, Yi,et al."Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria".BIOSENSORS & BIOELECTRONICS 55(2014):289-293.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Quaternized magnetic（1098KB）			限制开放	ODC PDDL	浏览