Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay

doi:10.1016/j.talanta.2009.06.057

	Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay
	Wan, Yi 1,2; Zhang, Dun 1; Hou, Baorong 1
	2009-11-15
发表期刊	TALANTA
ISSN	0039-9140
卷号	80 期号:1 页码:218-223
文章类型	Article
摘要	An impedimetric immunosensor was fabricated for rapid and non-labeled detection of sulfate-reducing bacteria, Desulforibrio caledoiensis (SRB) by immobilizing lectin-Concanavalin A using an agglutination assay. The immobilization of lectin was conducted using amine coupling on the surface of a gold (Au) electrode assembled with 11-Mercaptounclecanoic acid. Electrochemical impedance spectroscopy (EIS) was used to verify the stepwise assembly of the sensor system. The work conditions of the impedimetric immunosensor, such as pH of the buffer solutions and the incubation time of lectin, were optimized. Faradic impedance spectra for charge transfer for the redox probe Fe(CN)(6)(3-/4-) were measured to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (RI) increased with increasing SRB concentration. A linear relationship between R(ct) and SRB concentration was obtained in SRB concentration range of 1.8 to 1.8 x 10(7) cfu/ml. The variation of the SRB population during the growth process was also monitored using the impedimetric immunosensor. This approach has great potential for simple, low-cost. and time-saving monitoring of microbial populations. (C) 2009 Elsevier B.V. All rights reserved.; An impedimetric immunosensor was fabricated for rapid and non-labeled detection of sulfate-reducing bacteria, Desulforibrio caledoiensis (SRB) by immobilizing lectin-Concanavalin A using an agglutination assay. The immobilization of lectin was conducted using amine coupling on the surface of a gold (Au) electrode assembled with 11-Mercaptounclecanoic acid. Electrochemical impedance spectroscopy (EIS) was used to verify the stepwise assembly of the sensor system. The work conditions of the impedimetric immunosensor, such as pH of the buffer solutions and the incubation time of lectin, were optimized. Faradic impedance spectra for charge transfer for the redox probe Fe(CN)(6)(3-/4-) were measured to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (RI) increased with increasing SRB concentration. A linear relationship between R-ct and SRB concentration was obtained in SRB concentration range of 1.8 to 1.8 x 10(7) cfu/ml. The variation of the SRB population during the growth process was also monitored using the impedimetric immunosensor. This approach has great potential for simple, low-cost. and time-saving monitoring of microbial populations. (C) 2009 Elsevier B.V. All rights reserved.
关键词	Microbial Population Sulfate-reducing Bacteria Impedimetric Immunosensor Agglutination Assay Lectin
学科领域	Chemistry, Analytical
DOI	10.1016/j.talanta.2009.06.057
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000271055700033
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2272
专题	海洋环境腐蚀与生物污损重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wan, Yi,Zhang, Dun,Hou, Baorong. Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay[J]. TALANTA,2009,80(1):218-223.
APA	Wan, Yi,Zhang, Dun,&Hou, Baorong.(2009).Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay.TALANTA,80(1),218-223.
MLA	Wan, Yi,et al."Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay".TALANTA 80.1(2009):218-223.

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