Two novel antimicrobial peptides P 33-57 and mP 168-187 from zebrafish showing potent antibacterial activities

doi:10.1016/j.fsi.2024.109950

	Two novel antimicrobial peptides P 33-57 and mP 168-187 from zebrafish showing potent antibacterial activities
	Guo, Dongqiu 1; Wang, Hao 1,2; He, Jing 1; Zhang, Liqiao 1; Liu, Longxiao 1; Wang, Xia1
	2024-11-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	154 页码:10
通讯作者	Wang, Xia([email protected])
摘要	It is known that overuse or abuse of antibiotics has undoubtedly accelerated the global antibiotic resistance crisis, and long-time use of antibiotics may have adverse effects on the health of animal, human, and ecosystem. Therefore, it is necessary to find antibiotic alternatives that can be used in aquaculture and are non-toxic to the human. Here we clearly demonstrated that both the PH and FYVE domain of Plekhf2 in zebrafish have antibacterial properties and can interact with PGN in this study. Therefore, we screened four candidate peptides from the two domains. It was demonstrated that P 152-172 and P 168-187 had no obvious antibacterial activities, while P 33- 57 and mP 168-187 had strong antibacterial activities, which may be used as antimicrobial peptides. Additionally, transmission electron microscopy experiment revealed that P 33-57 and mP 168-187 can destroy the cell wall of bacteria, thereby directly killing bacteria. Importantly, it was found that P 33-57 and mP 168-187 had no hemolysis to red blood cells and lacked cytotoxicity. In summary, P 33-57 and mP 168-187 could be seen as antibacterial activity centers of PLEKHF2 and may be promising antimicrobial peptides to combat bacterial infections facing an antibiotic-resistance crisis.
关键词	Plekhf2 PH domain FYVE domain Candidate peptides Antibacterial activities
DOI	10.1016/j.fsi.2024.109950
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001338834600001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/199550
专题	海洋生物分类与系统演化实验室
通讯作者	Wang, Xia
作者单位	1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Guo, Dongqiu,Wang, Hao,He, Jing,et al. Two novel antimicrobial peptides P 33-57 and mP 168-187 from zebrafish showing potent antibacterial activities[J]. FISH & SHELLFISH IMMUNOLOGY,2024,154:10.
APA	Guo, Dongqiu,Wang, Hao,He, Jing,Zhang, Liqiao,Liu, Longxiao,&Wang, Xia.(2024).Two novel antimicrobial peptides P 33-57 and mP 168-187 from zebrafish showing potent antibacterial activities.FISH & SHELLFISH IMMUNOLOGY,154,10.
MLA	Guo, Dongqiu,et al."Two novel antimicrobial peptides P 33-57 and mP 168-187 from zebrafish showing potent antibacterial activities".FISH & SHELLFISH IMMUNOLOGY 154(2024):10.