Respiration rate and intestinal microbiota as promising indicators for assessing starvation intensity and health status in Pacific oyster (Crassostrea gigas)

doi:10.1016/j.aquaculture.2024.741330

IOCAS-IR > 实验海洋生物学重点实验室

	Respiration rate and intestinal microbiota as promising indicators for assessing starvation intensity and health status in Pacific oyster (Crassostrea gigas)
	Liu, Mingkun 1,2,3,5,6,9,10; Wei, Chenchen 2,3; Tan, Lintao 8; Xu, Wenwen 8; Li, Li 2,3,4,6,7,9,10; Zhang, Guofan 2,3,5,6,7,9,10
	2024-12-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	593 页码:12
通讯作者	Li, Li([email protected])
摘要	Implementing early disease warning is vital to prevent large-scale mortality of mollusks cultured in open waters. Starvation-induced emaciation syndrome is an undeniable prerequisite for mollusk mortality. Nevertheless, the indicators of mollusk response to starvation remain poorly understood. In this study, the effects of a 44-day starvation period on the physiological performance and symbiotic microbiota of oysters were investigated. Starvation resulted in reduced growth and survival and notable damage to intestinal structure, including shrinking lumens and shortened intestinal walls and villi. Analyses of the respiration rate and intestinal microbiota, including OTUs, taxonomy, and predicted functions, revealed a stepped change pattern with a tipping point on day 10, indicating that oysters employed distinct strategies to adapt to prolonged starvation. Biomarkers associated with nutrient digestion and uptake, such as Firmicutes and Staphylococcus abundance, as well as the Firmicutes/Bacteroidetes ratio, progressively declined with increasing starvation. Conversely, Proteobacteria, an indicator of energy disequilibrium and unstable gut microbiota, and pathogenic Vibrio, showed significant enrichment. As starvation progressed, bacterial competition intensified, as evidenced by the reduced network connectivity and the increasing percentage of negative correlations. Functional predictions demonstrated an early-stage enrichment of degradation functions followed by later-stage suppression during prolonged starvation. In contrast, biosynthesis functions exhibited the opposite trend, suggesting that intestinal microbiota adjusted their specific functions to cope with food deprivation. Collectively, a conceptual model of starvation stress tolerance limits (SSTL) was introduced to define critical thresholds and candidate markers for evaluating starvation intensity and oyster health. This study offers valuable insights into non-feeding mollusks' adaptation to prolonged starvation and provides potential indicators for mollusk mortality events.
关键词	Intestinal microbiota Starvation Warning model Mortality Respiration rate
DOI	10.1016/j.aquaculture.2024.741330
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2022YFD2400304]; Key Research and Development Program of Shandong[2022LZGC015]; Key Research and Development Program of Shandong[ZFJH202309]; Earmarked Fund for CARS[CARS-49]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001339200300001
出版者	ELSEVIER
WOS关键词	GUT MICROBIOTA ; STRESS ; GROWTH ; DEPRIVATION ; PHYSIOLOGY ; RESPONSES ; IMMUNITY ; ECOLOGY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/199535
专题	实验海洋生物学重点实验室
通讯作者	Li, Li
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315832, Peoples R China 2.Chinese Acad Sci, CAS, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 5.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 6.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Rushan Marine Econ & Dev Ctr, Rushan 264599, Peoples R China 9.Oyster Seed Ind, Shandong Technol Innovat Ctr, Qingdao 266071, Peoples R China 10.Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Zhanjiang 524006, Peoples R China
推荐引用方式 GB/T 7714	Liu, Mingkun,Wei, Chenchen,Tan, Lintao,et al. Respiration rate and intestinal microbiota as promising indicators for assessing starvation intensity and health status in Pacific oyster (Crassostrea gigas)[J]. AQUACULTURE,2024,593:12.
APA	Liu, Mingkun,Wei, Chenchen,Tan, Lintao,Xu, Wenwen,Li, Li,&Zhang, Guofan.(2024).Respiration rate and intestinal microbiota as promising indicators for assessing starvation intensity and health status in Pacific oyster (Crassostrea gigas).AQUACULTURE,593,12.
MLA	Liu, Mingkun,et al."Respiration rate and intestinal microbiota as promising indicators for assessing starvation intensity and health status in Pacific oyster (Crassostrea gigas)".AQUACULTURE 593(2024):12.