Environmental DNA sheds new insight on molecular adaptation of foraminifera to temperature from laboratory-controlled culture experiment

doi:10.1002/ece3.70243

	Environmental DNA sheds new insight on molecular adaptation of foraminifera to temperature from laboratory-controlled culture experiment
	Li, Haotian 1; Lei, Yanli1,2,3,4 ; Fa, Wenlong 1,4; Wu, Tianzhen 1,4; Li, Tiegang5
	2024-10-01
发表期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
卷号	14 期号:10 页码:10
通讯作者	Lei, Yanli([email protected]) ; Li, Tiegang([email protected])
摘要	Foraminifera is the most important temperature proxy of the ocean on long time scales. However, the absence of temperature-controlled experiments at different water depths hinders the advancement of paleotemperature reconstruction with foraminifera from the continental shelf. For the first time, this study investigated the response of benthic foraminifera to temperature change using microcosm culture and metabarcoding. Foraminiferal communities from three continental stations at varying water depths (6.0, 9.2, and 26.0 m) were cultured under five temperature gradients (6, 12, 18, 24, and 30 degrees C), with each treatment performed in triplicate. The foraminifera were fed with microalgae every 4 days, and the filtered seawater (through 0.22 mu m pores), acting as a medium, was changed accordingly. The experiment lasted for 80 days, and 47 DNA samples were obtained and analyzed, including three in situ samples. The results showed that foraminifera adjusted its growth rate within the low-temperature range and adopted an r-strategy to cope with high-temperature stress. In addition, the foraminifera from deeper water stations exhibited a pronounced vulnerability to diminishing read counts. The read counts, operational taxonomic units (OTU) counts and Margalef index of foraminifera and the read counts of Rotaliida exhibited a remarkably positive correlation with temperature. The recommended relationships were described as read counts = 1314.75T + 44754.51; OTU counts = 1.13T + 44.26; Margalef index =1.13*T + 44.26. This study established the first quantitative relationship between temperature and foraminifera molecular parameters that holds significant implications for long-time paleotemperature calibration in climate change.
关键词	benthic foraminifera laboratory-controlled culture molecular adaptation paleotemperature reconstruction
DOI	10.1002/ece3.70243
收录类别	SCI
语种	英语
资助项目	The National Natural Science Foundation of China[U1906211]; The National Natural Science Foundation of China[LSKJ202204200]; The National Natural Science Foundation of China[LSKJ202204900]; National Natural Science Foundation of China[XDB42000000]; Strategic Priority Research Program of the Chinese Academy of Sciences
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Ecology ; Evolutionary Biology
WOS记录号	WOS:001335703700001
出版者	WILEY
WOS关键词	GLOBAL OCEAN CARBONATE ; BENTHIC FORAMINIFERA ; SEA ; IDENTIFICATION ; BIODIVERSITY ; COMMUNITIES ; SEQUENCES ; RESPONSES ; SVALBARD ; CALCITE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/199475
专题	海洋生物分类与系统演化实验室
通讯作者	Lei, Yanli; Li, Tiegang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.MNR, Inst Oceanog 1, Key Lab Marine Sedimentol & Environm Geol, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Li, Haotian,Lei, Yanli,Fa, Wenlong,et al. Environmental DNA sheds new insight on molecular adaptation of foraminifera to temperature from laboratory-controlled culture experiment[J]. ECOLOGY AND EVOLUTION,2024,14(10):10.
APA	Li, Haotian,Lei, Yanli,Fa, Wenlong,Wu, Tianzhen,&Li, Tiegang.(2024).Environmental DNA sheds new insight on molecular adaptation of foraminifera to temperature from laboratory-controlled culture experiment.ECOLOGY AND EVOLUTION,14(10),10.
MLA	Li, Haotian,et al."Environmental DNA sheds new insight on molecular adaptation of foraminifera to temperature from laboratory-controlled culture experiment".ECOLOGY AND EVOLUTION 14.10(2024):10.