Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase

doi:10.3390/ijms252111667

IOCAS-IR > 实验海洋生物学重点实验室

	Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase
	Niu, Suhao 1,2,5; Qi, Lei 4; Zhang, Xiaoyue 1,2,5; He, Dongfang 4; Li, Pengwei 1,2; Wang, Hao 1,2; Bi, Yunchen 1,2,3,5
	2024-11-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	25 期号:21 页码:12
通讯作者	Wang, Hao([email protected]) ; Bi, Yunchen([email protected])
摘要	Chitin, an extracellular polysaccharide, is synthesized by membrane-embedded chitin synthase (CHS) utilizing intracellular substrates. The mechanism of the translocation of synthesized chitin across the membrane to extracellular locations remains unresolved. We prove that the chitin synthase from Phytophthora sojae (PsCHS) is a processive glycosyltransferase, which can rapidly produce and tightly bind with the highly polymerized chitin. We further demonstrate that PsCHS is a bifunctional enzyme, which is necessary and sufficient to translocate the synthesized chitin. PsCHS was purified and then reconstituted into proteoliposomes (PLs). The nascent chitin is generated and protected from chitinase degradation unless detergent solubilizes the PLs, showing that PsCHS translocates the newly produced chitin into the lumen of the PLs. We also attempted to resolve the PsCHS structure of the synthesized chitin-bound state, although it was not successful; the obtained high-resolution structure of the UDP/Mn2+-bound state could still assist in describing the characterization of the PsCHS's transmembrane channel. Consistently, we demonstrate that PsCHS is indispensable and capable of translocating chitin in a process that is tightly coupled to chitin synthesis.
关键词	chitin synthase membrane translocation processive glycosyltransferase
DOI	10.3390/ijms252111667
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China; National Natural Science Foundation of China[42376136]; Opening fund of State Key Laboratory of Silkworm Genome Biology[SKLSGB-ORP202105]; Instrument Improvement Funds of Shandong University Public Technology Platform[ts20230204]; Shandong Provincial Natural Science Foundation[ZR2024ZD32]; Pre-Research Project of Major Scientific Facilities in Shandong Province[DKXZZ202205]; [2023YFE0125400]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001351234100001
出版者	MDPI
WOS关键词	MEMBRANE TRANSLOCATION ; LENGTH CONTROL ; MECHANISM ; GLYCOSYLTRANSFERASES ; CLASSIFICATION ; BIOSYNTHESIS ; HYALURONAN ; MODEL
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/199394
专题	实验海洋生物学重点实验室
通讯作者	Wang, Hao; Bi, Yunchen
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Shandong Univ, Biomed Res Ctr Struct Anal, Jinan 250012, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Niu, Suhao,Qi, Lei,Zhang, Xiaoyue,et al. Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(21):12.
APA	Niu, Suhao.,Qi, Lei.,Zhang, Xiaoyue.,He, Dongfang.,Li, Pengwei.,...&Bi, Yunchen.(2024).Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(21),12.
MLA	Niu, Suhao,et al."Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.21(2024):12.