The Evolution and Biological Activity of Metazoan Mixed Lineage Kinase Domain-Like Protein (MLKL)

doi:10.3390/ijms251910626

IOCAS-IR > 实验海洋生物学重点实验室

	The Evolution and Biological Activity of Metazoan Mixed Lineage Kinase Domain-Like Protein (MLKL)
	Wang, Qingyue 1,2,3; Yuan, Zihao1,2 ; Xu, Hang1,2 ; Chen, Yuan 1,2,3; Sun, Li 1,2,3
	2024-10-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	25 期号:19 页码:12
通讯作者	Yuan, Zihao([email protected]) ; Sun, Li([email protected])
摘要	In mammals, mixed lineage kinase domain-like protein (MLKL) is the executor of necroptosis. MLKL comprises an N-terminal domain (NTD), which alone suffices to trigger necroptosis by forming pores in the plasma membrane, and a C-terminal domain that inhibits the NTD activity. Evolutionarily, MLKL is poorly conserved in animals and not found in Protostomia. Although MLKL orthologs exist in invertebrate Deuterostomia, the biological activity of invertebrate MLKL is unknown. Herein, we examined 34 metazoan phyla and detected MLKL not only in Deuterostomia but also in Protostomia (Rotifera). The Rotifera MLKL exhibited low identities with non-Rotifera MLKL but shared relatively high identities with non-metazoan MLKL. In invertebrates, MLKL formed two phylogenetic clades, one of which was represented by Rotifera. In vertebrates, MLKL expression was tissue-specific and generally rich in immune organs. When expressed in human cells, the MLKL-NTD of Rotifera, Echinodermata, Urochordata, and Cephalochordata induced strong necroptosis. The necroptotic activity of Rotifera MLKL depended on a number of conserved residues. Together these findings provided new insights into the evolution of MLKL in Metazoa and revealed the biological activity of invertebrate MLKL.
关键词	MLKL necroptosis evolution
DOI	10.3390/ijms251910626
收录类别	SCI
语种	英语
资助项目	Science & Technology Innovation Project of Laoshan Laboratory; Science Fund Program for Distinguished Young Scholars of Shandong Province (Overseas)[2022HWYQ-087]; Innovation Research Group Project of the National Natural Science Foundation of China[42221005]; Chinese Academy of Sciences; [LSKJ202203000]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001332405200001
出版者	MDPI
WOS关键词	PROGRAMMED NECROSIS ; NECROPTOSIS ; INFLAMMATION ; RIP3 ; DOWNSTREAM ; DIVERGENCE ; ACTIVATION ; BINDING ; FAMILY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/199292
专题	实验海洋生物学重点实验室
通讯作者	Yuan, Zihao; Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266404, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao 266404, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qingyue,Yuan, Zihao,Xu, Hang,et al. The Evolution and Biological Activity of Metazoan Mixed Lineage Kinase Domain-Like Protein (MLKL)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(19):12.
APA	Wang, Qingyue,Yuan, Zihao,Xu, Hang,Chen, Yuan,&Sun, Li.(2024).The Evolution and Biological Activity of Metazoan Mixed Lineage Kinase Domain-Like Protein (MLKL).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(19),12.
MLA	Wang, Qingyue,et al."The Evolution and Biological Activity of Metazoan Mixed Lineage Kinase Domain-Like Protein (MLKL)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.19(2024):12.