Molecular diversity and seasonal dynamics of <i>Ostreococcus</i> (Mamiellophyceae, Chlorophyta) in typical mariculture bays based on metabarcoding analysis

doi:10.1016/j.marenvres.2024.106764

	*Molecular diversity and seasonal dynamics of Ostreococcus* (Mamiellophyceae, Chlorophyta) in typical mariculture bays based on metabarcoding analysis**
	Liu, Feng 1,2,3,4; Li, Jiamin 1,2,4; Jin, Shuangle 1,2,3,4; Liu, Liang 5
	2024-11-01
发表期刊	MARINE ENVIRONMENTAL RESEARCH
ISSN	0141-1136
卷号	202 页码:11
通讯作者	Liu, Feng([email protected])
摘要	Ostreococcus (Mamiellophyceae, Chlorophyta) is a cosmopolitan genus of marine pico-phytoplankton and the smallest free-living photosynthetic eukaryotes with cell size of 1-2 mu m. To understand the diversity and spatiotemporal distribution of Ostreococcus in the Rongcheng coastal regions in northern China, metabarcoding analysis based on the 18S rDNA V4 molecular marker was applied to study the molecular diversity and seasonal dynamics of Ostreococcus in three typical mariculture bays (Rongcheng Bay, Lidao Bay and Sanggou Bay). A total of 103 amplicon sequence variants (ASVs) annotated as Ostreococcus were detected in these three typical mariculture bays throughout the year. The top five ASVs in terms of abundance were ASV4, ASV9, ASV14, ASV28 and ASV109, totally occupying 99.1% of Ostreococcus reads. Phylogenetic analysis showed that these five dominant ASVs represented two Ostreococcus ecotypes (OI and OII) and were grouped into four Ostreococcus clades including Ostreococcus lucimarinus (ASV9) and Ostreococcus tauri (ASV28 and ASV109) in OI, and Ostreococcus sp. RC1 (ASV4) and Ostreococcus sp. RC2 (ASV14) in OII, which provided direct evidence to support the co-existence of two ecotypes in the Rongcheng coastal regions. Five dominant ASVs in OI and OII exhibited two distinct seasonal distribution patterns. Three dominant ASVs (ASV9, ASV28 and ASV109) in OI could be detected in all four seasons of the year, exhibiting native distribution properties, while two ASVs (ASV4 and ASV14) in OII decreased sharply in winter and could not be detected in spring, exhibiting characteristics of alien inputs. The composition, succession and association of Ostreococcus community were mainly driven by water temperature in these mariculture bays. This study helps us systematically understand the molecular diversity and distribution patterns of Ostreococcus in typical mariculture bays in northern China, laying the foundation for understanding and revealing the ecological functions of pico-phytoplankton.
关键词	Pico-phytoplankton Ostreococcus Typical mariculture bays Seasonal dynamics Molecular diversity
DOI	10.1016/j.marenvres.2024.106764
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42276133]; Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050302]; Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050403]; Rongcheng Ocean and Fishery Monitoring and Disaster Mitigation Center; Rongcheng Science and Technology Bureau; Rongcheng Ocean Development Bureau
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology ; Toxicology
WOS记录号	WOS:001327611700001
出版者	ELSEVIER SCI LTD
WOS关键词	PHYTOPLANKTON ; ABUNDANCE ; PICOPHYTOPLANKTON ; ECOTYPES ; PLANKTON ; VIRUSES
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/199280
专题	海洋生态与环境科学重点实验室
通讯作者	Liu, Feng
作者单位	1.Chinese Acad Sci IOCAS, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266000, Shandong, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 101408, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266000, Shandong, Peoples R China 5.Rongcheng Ocean & Fishery Monitoring & Disaster Mi, Rongcheng 264300, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Feng,Li, Jiamin,Jin, Shuangle,et al. Molecular diversity and seasonal dynamics of Ostreococcus (Mamiellophyceae, Chlorophyta) in typical mariculture bays based on metabarcoding analysis[J]. MARINE ENVIRONMENTAL RESEARCH,2024,202:11.
APA	Liu, Feng,Li, Jiamin,Jin, Shuangle,&Liu, Liang.(2024).Molecular diversity and seasonal dynamics of Ostreococcus (Mamiellophyceae, Chlorophyta) in typical mariculture bays based on metabarcoding analysis.MARINE ENVIRONMENTAL RESEARCH,202,11.
MLA	Liu, Feng,et al."Molecular diversity and seasonal dynamics of Ostreococcus (Mamiellophyceae, Chlorophyta) in typical mariculture bays based on metabarcoding analysis".MARINE ENVIRONMENTAL RESEARCH 202(2024):11.