Bacterial killing and the dimensions of bacterial death

doi:10.1038/s41522-024-00559-9

IOCAS-IR

	Bacterial killing and the dimensions of bacterial death
	Wu, Renfei 1,2,3; Li, Cong 1,2,4; Li, Jiuyi 5; Sjollema, Jelmer 1,2; Geertsema-Doornbusch, Gesinda I.1,2; de Haan-Visser, H. Willy 1,2; Dijkstra, Emma S. C.1,2; Ren, Yijin 6,7; Zhang, Zexin 4; Liu, Jian 3; Flemming, Hans C.8,9; Busscher, Henk J.1,2; van der Mei, Henny C.1,2
	2024-09-17
发表期刊	NPJ BIOFILMS AND MICROBIOMES
卷号	10 期号:1 页码:8
通讯作者	van der Mei, Henny C.([email protected])
摘要	Bacteria can be dead, alive, or exhibit slowed or suspended life forms, making bacterial death difficult to establish. Here, agar-plating, microscopic-counting, SYTO9/propidium-iodide staining, MTT-conversion, and bioluminescence-imaging were used to determine bacterial death upon exposure to different conditions. Rank correlations between pairs of assay outcomes were low, indicating different assays measure different aspects of bacterial death. Principal-component analysis yielded two principal components, named "reproductive-ability" (PC1) and "metabolic-activity" (PC2). Plotting of these principal components in two-dimensional space revealed a dead region, with borders defined by the PC1 and PC2 values. Sensu stricto implies an unpractical reality that all assays determining PC1 and PC2 must be carried out in order to establish bacterial death. Considering this unpracticality, it is suggested that at least one assay determining reproductive activity (PC1) and one assay determining metabolic activity (PC2) should be used to establish bacterial death. Minimally, researchers should specifically describe which dimension of bacterial death is assessed, when addressing bacterial death.
DOI	10.1038/s41522-024-00559-9
收录类别	SCI
语种	英语
资助项目	Universitair Medisch Centrum Groningen (University Medical Center Groningen); University Medical Center Groningen, The Netherlands
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:001314953400001
出版者	NATURE PORTFOLIO
WOS关键词	BIOLUMINESCENCE ; IMAGEJ
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/198980
专题	中国科学院海洋研究所
通讯作者	van der Mei, Henny C.
作者单位	1.Univ Groningen, Antonius Deusinglaan 1, NL-9713 AV Groningen, Netherlands 2.Univ Med Ctr Groningen, Dept Biomat & Biomed Technol, Dept Biomed Engn, Antonius Deusinglaan 1, NL-9713 AV Groningen, Netherlands 3.Soochow Univ, Inst Funct Nano & Soft Mat, Jiangsu Key Lab Carbon Based Funct Mat & Devices, Joint Int Res Lab Carbon Based Funct Mat & Devices, 199 Renai Rd, Suzhou 215123, Jiangsu, Peoples R China 4.Soochow Univ, Coll Chem Chem Engn & Mat Sci, 199 Renai Rd, Suzhou 215123, Jiangsu, Peoples R China 5.Beijing Jiaotong Univ, Sch Environm, Beijing 100044, Peoples R China 6.Univ Groningen, Hanzepl 1, NL-9700 RB Groningen, Netherlands 7.Univ Med Ctr Groningen, Dept Orthodont, Hanzepl 1, NL-9700 RB Groningen, Netherlands 8.Univ Duisburg Essen, Fac Chem, Biofilm Ctr, Univ Str 5, D-45141 Essen, Germany 9.Chinese Acad Sci IOCAS, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wu, Renfei,Li, Cong,Li, Jiuyi,et al. Bacterial killing and the dimensions of bacterial death[J]. NPJ BIOFILMS AND MICROBIOMES,2024,10(1):8.
APA	Wu, Renfei.,Li, Cong.,Li, Jiuyi.,Sjollema, Jelmer.,Geertsema-Doornbusch, Gesinda I..,...&van der Mei, Henny C..(2024).Bacterial killing and the dimensions of bacterial death.NPJ BIOFILMS AND MICROBIOMES,10(1),8.
MLA	Wu, Renfei,et al."Bacterial killing and the dimensions of bacterial death".NPJ BIOFILMS AND MICROBIOMES 10.1(2024):8.