CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost

doi:10.1016/j.ijbiomac.2024.135265

IOCAS-IR > 实验海洋生物学重点实验室

	CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost
	Wu, Meng 1,2,3,4; Chen, Yuan 1,2,3,4; Yuan, Zihao1,2,3,4 ; Xu, Hang1,2,3 ; Sun, Li 1,2,3,4
	2024-11-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	279 页码:9
通讯作者	Xu, Hang([email protected]) ; Sun, Li([email protected])
摘要	Caspase 9 (CASP9) is a well-known initiator caspase of intrinsic apoptosis. In humans, cIAP1 binds and induces degradation of the activated form of CASP9, but not pro-CASP9. In fish, the activity and regulation of CASP9 remain unknown. In this work, using flounder Paralichthys olivaceus as a representative species, we examined the regulatory mechanism of CASP9 in teleost. P. olivaceus CASP9 (PoCASP9) induced robust apoptosis, which was inhibited by P. olivaceus cIAP1 (PocIAP1). Unlike human cIAP1, PocIAP1 bound both proand active PoCASP9 and induced their degradation via the RING domain-involved proteasome pathway. In humans, the adaptor molecule CRADD cannot interact with CASP9. In contrast, P. olivaceus CRADD (PoCRADD) bound both proand active PoCASP9 via CARD-CARD interaction and enhanced apoptosis by promoting the cellular levels of proand active PoCASP9. Furthermore, PoCRADD abrogated the inhibition of PoCASP9 by PocIAP1 by preventing PocIAP1-PoCASP9 interaction. Together these results reveal a CASP9 regulation mechanism in teleost that differs from that in humans and demonstrate that teleost CASP9 is tightly and directly controlled by both negative and positive regulators that exert a regulation effect both before and after CASP9 activation. These findings advance our understanding of the regulation of CASP9-mediated apoptosis in vertebrates.
关键词	Caspase cIAP CRADD Apoptosis Regulation
DOI	10.1016/j.ijbiomac.2024.135265
收录类别	SCI
语种	英语
资助项目	Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000]; National Natural Science Foundation of China[31730100]; Postdoctoral Fellowship Program of China Postdoctoral Science Foundation[GZC20232709]; National Key Research and Development Program of China[2018YFD0900500]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001309080800001
出版者	ELSEVIER
WOS关键词	MOLECULAR-CLONING ; CYTOCHROME-C ; CELL-DEATH ; ACTIVATION ; MECHANISMS ; BINDING ; RAIDD ; GENE ; XIAP
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/198654
专题	实验海洋生物学重点实验室
通讯作者	Xu, Hang; Sun, Li
作者单位	1.Chinese Acad Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wu, Meng,Chen, Yuan,Yuan, Zihao,et al. CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,279:9.
APA	Wu, Meng,Chen, Yuan,Yuan, Zihao,Xu, Hang,&Sun, Li.(2024).CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,279,9.
MLA	Wu, Meng,et al."CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 279(2024):9.