Selection of protocols for phytoplankton pigment analysis: a comparative study

doi:10.1007/s00343-024-4025-9

	Selection of protocols for phytoplankton pigment analysis: a comparative study
	Wang, Jinxiu1,2 ; Kong, Fanzhou1,2,3 ; Niu, Zhuang 4; Yu, Rencheng 1,2,3
	2024-09-14
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	14
通讯作者	Kong, Fanzhou([email protected])
摘要	Pigments are widely used as indices for estimation of phytoplankton biomass and composition, and many protocols have been developed to analyze pigments in phytoplankton. Different protocols were compared using four solvents (methanol, 95% methanol, dimethylformamide, and 90% acetone) and two instruments (fluorometer and high-performance liquid chromatography (HPLC) coupled with diode array detector). Analysis of chlorophyll a (Chl a) with fluorometer could lead to over- or underestimation due to the interference from its derivatives in all probability. Among the four extractants, 90% acetone had a high recovery for chlorophylls. In contrast, 95% methanol was a poor extractant for chlorophylls due to the degradation of Chl a, especially in diatoms. The 95% methanol, however, had high extraction efficiencies for most diagnostic xanthophylls. Therefore, the selection of pigment analytical protocols should follow the specific purpose of phytoplankton study. In addition to fluorometry, an HPLC method with 90% acetone as extractant shall be a good choice for the analysis of Chl a to estimate phytoplankton biomass, especially for diatom-dominated samples, while an HPLC method with 95% methanol as extractant be more suitable to characterize different taxa in phytoplankton communities.
关键词	pigment fluorometry high-performance liquid chromatography (HPLC) extraction phytoplankton
DOI	10.1007/s00343-024-4025-9
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:001313441000005
出版者	SCIENCE PRESS
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; CHLOROPHYLL-A CONCENTRATIONS ; PHOTOSYNTHETIC PIGMENTS ; MARINE-PHYTOPLANKTON ; EXTRACTION METHODS ; ALGAL PIGMENTS ; CAROTENOIDS ; DEGRADATION ; PRODUCTS ; CHEMTAX
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/198634
专题	海洋生态与环境科学重点实验室
通讯作者	Kong, Fanzhou
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266214, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Marine Monitoring & Forecasting Ctr Zhejiang Prov, Hangzhou 310007, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jinxiu,Kong, Fanzhou,Niu, Zhuang,et al. Selection of protocols for phytoplankton pigment analysis: a comparative study[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:14.
APA	Wang, Jinxiu,Kong, Fanzhou,Niu, Zhuang,&Yu, Rencheng.(2024).Selection of protocols for phytoplankton pigment analysis: a comparative study.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14.
MLA	Wang, Jinxiu,et al."Selection of protocols for phytoplankton pigment analysis: a comparative study".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):14.