Haemolysins are essential to the pathogenicity of deep-sea<i> Vibrio</i><i> fluvialis</i>

doi:10.1016/j.isci.2024.109558

IOCAS-IR > 实验海洋生物学重点实验室

	Haemolysins are essential to the pathogenicity of deep-sea Vibrio fluvialis
	Wang, Yujian1,2,3 ; Luo, Jingchang1,2,3,4 ; Zhao, Yan5 ; Zhang, Jian 6; Guan, Xiaolu 1,2,3; Sun, Li 1,2,3,4
	2024-05-17
发表期刊	ISCIENCE
卷号	27 期号:5 页码:15
通讯作者	Zhao, Yan([email protected]) ; Sun, Li([email protected])
摘要	Vibrio fluvialis is an emerging foodborne pathogen that produces VFH ( Vibrio fluvialis hemolysin) and BVFH (delta-Vibrio Vibrio fluvialis hemolysin). The function of BVFH is unclear. Currently, no pathogenic V . fluvialis from deep sea has been reported. In this work, a deep-sea V . fluvialis isolate (V13) was examined for pathogenicity. V13 was most closely related to V. fluvialis ATCC 33809, a human isolate, but possessed 262 unique genes. V13 caused lethal infection in fish and induced pyroptosis involving activation of the NLRP3 inflammasome, caspase 1 (Casp1), and gasdermin D (GSDMD). V13 defective in VFH or VFH plus BVFH exhibited significantly weakened cytotoxicity. Recombinant BVFH induced NLRP3-Casp1GSDMD-mediated pyroptosis in a manner that depended on K+ + efflux and intracellular Ca2+ 2+ accumulation. BVFH bound several plasma membrane lipids, and these bindings were crucial for BVFH cytotoxicity. Together these results provided new insights into the function of BVFH and the virulence mechanism of V. fluvialis. .
DOI	10.1016/j.isci.2024.109558
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050402]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403]; Innovation Research Group Project of the National Natural Science Foundation of China[42221005]; Shandong Postdoctoral Science Foundation[SDCX-ZG-202303040]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001290486700001
出版者	CELL PRESS
WOS关键词	NLRP3 INFLAMMASOME ; SP NOV. ; CHOLERAE ; INFECTIONS ; PYROPTOSIS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/197572
专题	实验海洋生物学重点实验室
通讯作者	Zhao, Yan; Sun, Li
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100049, Peoples R China 5.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Sch Med, Beijing 100084, Peoples R China 6.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yujian,Luo, Jingchang,Zhao, Yan,et al. Haemolysins are essential to the pathogenicity of deep-sea Vibrio fluvialis[J]. ISCIENCE,2024,27(5):15.
APA	Wang, Yujian,Luo, Jingchang,Zhao, Yan,Zhang, Jian,Guan, Xiaolu,&Sun, Li.(2024).Haemolysins are essential to the pathogenicity of deep-sea Vibrio fluvialis.ISCIENCE,27(5),15.
MLA	Wang, Yujian,et al."Haemolysins are essential to the pathogenicity of deep-sea Vibrio fluvialis".ISCIENCE 27.5(2024):15.