A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from <i>Lessonia trabeculata</i> and their bioactivities

doi:10.1007/s00343-024-4042-8

IOCAS-IR > 实验海洋生物学重点实验室

	*A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata* and their bioactivities**
	Yan, Changhui 1,2; Pan, Mingxuan 4; Geng, Lihua 2,3; Zhang, Quanbin 2,3; Hu, Yadong 4; Wang, Jing2,3 ; Ye, Sujuan 1
	2024-08-12
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	15
通讯作者	Hu, Yadong([email protected]) ; Wang, Jing([email protected])
摘要	Alginate oligosaccharides (AOS) and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds. We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from Lessonia trabeculata, and found that the optimum extraction procedure was: first, using 10-g/L Na2CO3 solution to digest the seaweeds for 2 h at 80 degrees C with the material-liquid ratio 1.20 (g/mL), and then adding 4.0% fucoidan cleavage enzyme for reaction for 12 h at 45 degrees C. The yield of AOS and fucoidan was 21.36% and 14.40%, respectively, which was higher than those using traditional methods. The degree of polymerization of AOS was determined to be 3-5, confirming the results through thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESI-MS). The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97. Through anion-exchange column chromatography, four sulfated polysaccharide fractions (F0.5, F1.0, F1.5, and F2.0) were successfully isolated from fucoidan. Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides, consisting mainly of mannuronic acid, fucose, and xylose, while the other fractions were predominantly composed of fucose and galactose. The sulfate group content increased from 8.74% to 18.76%, while the uronic acid content decreased from 23.79% to 5.58% across the fractions. All fractions of fucoidan exhibited significant antioxidant activity in various tests. There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals. Additionally, both AOS and the fucoidan fractions showed immunostimulation activity and were nontoxic to RAW264.7 cells. These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds. It has potential applications in the processing industry of brown seaweeds.
关键词	Lessonia trabeculata alkaline digestion-enzymatic hydrolysis alginate oligosaccharides fucoidan antioxidant activity
DOI	10.1007/s00343-024-4042-8
收录类别	SCI
语种	英语
资助项目	Jiangsu Coast Development Group Co., Ltd.; Marine Biological High Value Development and Utilization Project[2023YHTZZZ04]; Natural Science Foundation of Shandong Province[ZR2022MD007]; Science and Technology Project of Fujian Province[2022T3024]; Science and Technology Project of Fujian Province[2023T3057]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:001289957200001
出版者	SCIENCE PRESS
WOS关键词	SACCHARINA-JAPONICA ; SODIUM ALGINATE ; POLYSACCHARIDES ; ACID ; DEGRADATION ; SUGARS ; ALGAE ; LYASE
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/196531
专题	实验海洋生物学重点实验室
通讯作者	Hu, Yadong; Wang, Jing
作者单位	1.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Jiangsu Coast Dev Investment Co Ltd, Jiangsu Coast Dev Grp Co Ltd, Jiangsu Innovat Ctr Marine Bioresource, Nanjing 210019, Peoples R China
推荐引用方式 GB/T 7714	Yan, Changhui,Pan, Mingxuan,Geng, Lihua,et al. A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:15.
APA	Yan, Changhui.,Pan, Mingxuan.,Geng, Lihua.,Zhang, Quanbin.,Hu, Yadong.,...&Ye, Sujuan.(2024).A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,15.
MLA	Yan, Changhui,et al."A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):15.