Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye

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	Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye
	Li CP(李翠萍); Yu HH(于华华); Feng JH(冯金华); Chen XL(陈晓琳); Li PC(李鹏程)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2009-02-15
出版年	2009
卷号	v.27 期号:01 页码:174-178
文献类型	CNKI期刊论文
摘要	In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well.
关键词	concentration hemolytic activity Rhopilema esculentum Kishinouye venom
CNKI专辑号	A;E;
CNKI专辑名称	基础科学;医药卫生科技;
CNKI专题号	E079;
CNKI专题名称	药学;
分类号	R99
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the Award Foundation of Scientific Research for Excellent Young and Middle-age Scientist of Shandong Province (No. 2006BS07003) ; the Knowledge Innovation Project of Chinese Academy of Sciences (KZCX2-YW-R-104)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192694
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateSchooloftheChineseAcademyofSciences
推荐引用方式 GB/T 7714	Li CP,Yu HH,Feng JH,et al. Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye. 2009.