Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses

IOCAS-IR

	Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses
	You F(尤锋); Wang W(王伟); Xu DD(徐冬冬); Zhu XP(朱香萍); Ni J(倪静); Wu ZH(吴志昊); Xu YL(徐永立); Wang XC(王新成); Zhang PJ(张培军)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2009-05-15
出版年	2009
卷号	v.27 期号:02 页码:135-141
文献类型	CNKI期刊论文
摘要	The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.
关键词	artificial hybridization Paralichthys olivaceus (Temminck et Schlegel) Kareius bicoloratus (Basilewsky) karyotype allozyme RAPD
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by National Natural Science Foundation of China (No. 30571445) ; National High-Technology Research and Development Program (863 Program, No. 2006AA10A404) ; Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192596
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.QingdaoAgriculturalUniversity
推荐引用方式 GB/T 7714	You F,Wang W,Xu DD,et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses. 2009.