Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis

IOCAS-IR

	Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis
	Li YY(李艳燕); Shen SD(沈颂东); He LH(贺丽虹); Xu P(许璞); Wang GC(王广策)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2009-08-15
出版年	2009
卷号	v.27 期号:03 页码:75-83
文献类型	CNKI期刊论文
摘要	The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli(NB,PT and ST) were amplified,sequenced and analyzed.In addition,the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated.The results are as follows:the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains.The sequences of ITS1 were 331 bp to 334 bp,while those of the 5.8S rDNA were 158 bp and the sequences of ITS2 ranged from 673 bp to 681 bp.The sequences of the ITS had a high level of homology(up to 99.5%) with that of P.haitanensis(DQ662228) retrieved from GenBank,but were only approximately 50% homologous with those of other species of Porphyra.The results obtained when a phylogenetic tree was constructed coincided with the results of the homology analysis.These results suggest that the three cultivated strains of P.haitanensis evolved conservatively and that the ITS showed evolutionary consistency.However,the sequences of the ITS and 5.8S rDNA of different Porphyra species showed great variations.Therefore,the relationship of Porphyra interspecies phyletic evolution could be judged,which provides the proof for Porphyra identification study.However,proper classifications of the subspecies and the populations of Porphyra should be determined through the use of other molecular techniques to determine the genetic variability and rational phylogenetic relationships.
关键词	Porphyra haitanensis ITS region sequence analysis interspecies phylogeny
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.3
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China (No 40576074) ; the Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences (No KFN92007NO1)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192499
专题	中国科学院海洋研究所
作者单位	1.DepartmentofCellBiology,MedicalCollegeofSoochowUniversity 2.DepartmentofBiologyandFoodEngineering,ChangshuInstituteofTechnology 3.InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Li YY,Shen SD,He LH,et al. Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis. 2009.