Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum

IOCAS-IR

	Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum
	Han YF(韩一凡); Mo ZL(莫照兰); Mao YX(茅云翔); Xiao P(肖鹏); Li J(李杰); Hao B(郝斌); Guo DS(郭东升)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2009-08-15
出版年	2009
卷号	v.27 期号:03 页码:101-108
文献类型	CNKI期刊论文
摘要	Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58(62.4%) were homologous to known genes,30(32.3%) were genes with hypothetical functions,and the remaining 5(5.3%) were unknown genes.We demonstrated the utility of this library by PCR screening of 10 genes.This resulted in an average of 6.2 fosmid clones per screening.This fosmid library offers a new tool for gene screening and cloning of V.anguillarum,and for comparative genomic studies among Vibrio species.
关键词	end-sequencing fosmid library PCR screening Vibrio anguillarum
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S941
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National Basic Research Program of China (973 Program)(No 2006CB101803) ; the High Technology Research and development Program of China (863 Program)(No 2006AA6100310) ; the National Natural Science Foundation of China (No 30871935)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192498
专题	中国科学院海洋研究所
作者单位	1.OceanUniversityofChina 2.InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Han YF,Mo ZL,Mao YX,et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum. 2009.