Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

IOCAS-IR

	Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC
	Zhang JJ(张晶晶); Zhang QB(张全斌); Wang J(王晶); Shi XL(石学连); Zhang ZS(张忠山)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2009-08-15
出版年	2009
卷号	v.27 期号:03 页码:160-164
文献类型	CNKI期刊论文
摘要	We developed an HPLC method for analysis of the monosaccharide composition of fucoidans.The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid.Using ribose as the internal standard,the monosaccharide derivatives,obtained with 1-Phenyl-3-methyl-5-pyrazolone(PMP),were separated by reverse-phase HPLC using a gradient elution process,and monitored by ultraviolet detection at 245 nm.In the concentration range of 0.1-2.0 mmol/L,the peak area of each monosaccharide had a good linear relationship with its concentration(r2>0.998).The average recoveries of mannose,rhamnose,glucuronic acid,glucose,galactose,xylose,and fucose were 86.2%,95.1%,62.5%,102.0%,94.8%,66.6%,and 105.1%,respectively.This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.
关键词	fucoidan monosaccharide derivation HPLC
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006
CNKI专题名称	生物学;
分类号	Q946
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the Key Technology Research & Development Program of Shandong Province (No 2007GG1005007)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192485
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateSchoolofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Zhang JJ,Zhang QB,Wang J,et al. Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC. 2009.