Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides

IOCAS-IR

	Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
	Niu JF(牛建峰); Wang GC(王广策); Lv F(吕芳); Zhou BC(周百成); Peng G(彭光)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2009-08-15
出版年	2009
卷号	v.27 期号:03 页码:84-94
文献类型	CNKI期刊论文
摘要	A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was not a glycoprotein.The hemagglutinating activity(HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine,N-Acetylglucosamine,and the glycoprotein bovine submaxillary mucin.The lectin preferentially agglutinated Gram-negative bacterium.The HA of this lectin was stable between pH 4 to pH 10.Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution(0.5 mg ml-1).Our results suggest that the regeneration of B.hypnoides is mediated by this lectin.We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater(pH 8.0).Given that nigericin dissipates proton gradients across the membrane,we hypothesize that the aggregation of cell organelles was proton-gradient dependent.
关键词	Bryopsis hypnoides lectin hemagglutinating cell organelle aggregation regeneration
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006
CNKI专题名称	生物学;
分类号	Q948
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the Natural Science Foundation of China (Nos 40806063,30830015) ; the National High Technology Research and Development Program of China (863 Program) (Nos2007AA09Z406,2006AA10A413)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192484
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.CollegeofMarineScienceandEngineering,TianjinUniversityofScienceandTechnology 3.TheGraduateSchoolofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Niu JF,Wang GC,Lv F,et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides. 2009.