Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

IOCAS-IR

	Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease
	Mo ZL(莫照兰); Guo DS(郭东升); Mao YX(茅云翔); Ye XH(叶旭红); Zou YX(邹玉霞); Xiao P(肖鹏); Hao B(郝斌)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2010-02-15
出版年	2010
卷号	v.28 期号:01 页码:57-63
文献类型	CNKI期刊论文
摘要	We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In addition,the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes.Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish.We propose that prtV plays an important role in the pathogenesis of V.anguillarum.
关键词	Vibrio anguillarum prtV proteolysis hemolysis mucus LD50
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S941
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National Basic Research Program of China (973 Program) (No. 2006CB101803) ; the National Natural Science Foundation of China (No. 30871935) ; the National High Technology Research and Development Program (863 Program) (No. 2003AA622070)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192261
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences 3.OceanUniversityofChina
推荐引用方式 GB/T 7714	Mo ZL,Guo DS,Mao YX,et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease. 2010.