In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica

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	In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica
	Wang J(王晶); Zhang QB(张全斌); Zhang ZS(张忠山); Hou Y(侯赟); Zhang H(张虹)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2011-05-15
出版年	2011
卷号	v.29 期号:03 页码:189-195
文献类型	CNKI期刊论文
摘要	Fucoidan, a group of sulfated heteropolysaccharides, was extracted from Laminaria japonica, an important economic alga species in China. The anticoagulant activity of fucoidan and its derivatives (including sulfated, phosphorylated, and aminated fucoidan) was examined using in-vitro anticoagulant systems. The correlation between chemical variations within the fucoidan group and anticoagulant activity was determined. The in-vitro anticoagulant properties of fucoidan and its derivatives were determined by measuring activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicate anticoagulant activity in all samples using APTT and TT assays; however, only the fucoidan derivatives affected the PT assay. Thus, the fucoidan derivatives were able to inhibit both intrinsic and extrinsic blood coagulants. Fucoidan (FPS) and its derivatives presented better anticoagulant activity than low molecular weight fucoidan (DFPS) and its derivatives, suggesting that molecular weight and proper conformation are contributing factors for anticoagulant activity of polysaccharides. Amino groups have a positive charge and can thus change the charge density of fucoidan. Accordingly, among the tested samples, aminated fucoidan (NF) was the most active reflecting the importance of charge density for anticoagulant activity. Available data obtained using in-vitro models suggest that the sulfate content, sulfate/total-sugar ratio, molecular weight, and the substituted group of fucoidan are important factors for anticoagulant activity but that the influence of sulfate, phosphate and amino groups on anticoagulant activity was different.
关键词	fucoidan derivative Laminaria japonica anticoagulant activity
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.3
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the Ocean Public Welfare Scientific Research Project, State Oceanic Administration of the People's Republic of China (No. 201005024)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/191701
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Wang J,Zhang QB,Zhang ZS,et al. In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica. 2011.