The role of phosphatidylinositol signaling pathway in regulating serotonin-induced oocyte maturation in Mercenaria mercenaria

IOCAS-IR

	The role of phosphatidylinositol signaling pathway in regulating serotonin-induced oocyte maturation in Mercenaria mercenaria
	Wang Q(王清); Zhang T(张涛)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2011-05-15
出版年	2011
卷号	v.29 期号:03 页码:78-85
文献类型	CNKI期刊论文
摘要	Serotonin (5-HT) has been found to stimulate meiotic maturation of oocytes in many molluscs. During maturation, several signaling pathways are involved, especially the phosphatidylinositol and cAMP pathways. In order to examine the possible role of the phosphatidylinositol signaling pathway in regulating oocyte maturation in Mercenaria mercenaria, the effects of the activator/inhibitor of phospholipase (PLC) and protein kinase C (PKC) on serotonin-induced maturation were studied. Results show that high-concentrations of neomycin (inhibitor of PLC) blocked oocyte maturation, while 9, 10-dimethyl-1, 2-benzanthracene (DMBA, activator of PLC) promoted oocyte maturation in the presence of serotonin. It was also found that in the presence of serotonin, phorbol 12-myristate 13-acetate (PMA, activator of PKC) inhibited oocyte maturation, while sphingosine (inhibitor of PKC) stimulated oocyte maturation. These results indicate that serotonin-induced oocyte maturation requires the activation of the phosphatidylinositol pathway. Decrease of PLC inhibited serotonin-induced oocyte maturation, whereas a decrease of PKC stimulated the maturation. Thus, our study indicates that serotonin promotes maturation of M. mercenaria oocytes through PLC stimulated increase in calcium ion concentration via inositol-1, 4, 5-trisphosphate (IP3) but not PKC.
关键词	GVBD Mercenaria mercenaria oocyte maturation phosphatidylinositol signaling pathway
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National High Tecnhology Research and Development Program of China (863 Program) (No. 2004AA603810)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/191697
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Wang Q,Zhang T. The role of phosphatidylinositol signaling pathway in regulating serotonin-induced oocyte maturation in Mercenaria mercenaria. 2011.