Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3

IOCAS-IR

	Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3
	Ren SY(任世英); Li XQ(李相前); Jia JB(贾建波); Liu F(刘飞); Xiao T(肖天)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2011-09-15
出版年	2011
卷号	v.29 期号:05 页码:3-7
文献类型	CNKI期刊论文
摘要	Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.
关键词	Halomonas sp. YSR-3 D-hydantoinase polyphosphate-accumulating bacterium
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q93;Q78
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National Basic Research Program of China (973 Program) (No. 2006AA10A401) ; the National Natural Science Foundation of China (No. 40376048)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/191571
专题	中国科学院海洋研究所
作者单位	1.SchoolofLifeScienceandChemicalEngineering,HuaiyinInstituteofTechnology 2.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Ren SY,Li XQ,Jia JB,et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3. 2011.