Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation

IOCAS-IR

	Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation
	Cui YL(崔玉琳); Jiang P(姜鹏); Wang JF(王金锋); Li FC(李富超); Chen YJ(陈英杰); Zheng GT(郑国庭); Qin S(秦松)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2012-05-15
出版年	2012
卷号	v.30 期号:03 页码:113-117
文献类型	CNKI期刊论文
摘要	Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga.
关键词	bar glass-bead agitation nuclear transformation particle bombardment Platymonas subcordiformis
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q943
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National Basic Research Program of China (973 Program) (No. 2011CB200902) ; the National High Technology Research and Development Program of China (863 Program) (Nos. 2009AA10Z106,2009AA064401) ; the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. 2010BSB02009) ; the National Natural Science Foundation of China (Nos. 40876082,30870247) ; the Science and Technology Project of Qingdao City (No.09-1-3-59-jch)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/191255
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences 3.KeyLaboratoryofMarineBiotechnology,NingboUniversity
推荐引用方式 GB/T 7714	Cui YL,Jiang P,Wang JF,et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation. 2012.