Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

IOCAS-IR

	Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
	Wang DD(王冬冬); Li FH(李富花); Li SH(李诗豪); Wen R(温荣); Xiang JH(相建海)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2012-07-15
出版年	2012
卷号	v.30 期号:04 页码:107-115
文献类型	CNKI期刊论文
摘要	Antimicrobial peptides (AMPs), as key immune effectors, play important roles in the innate immune system of invertebrates. Different types of AMPs, including Penaeidin, Crustin, ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however, systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study, we analyzed the expression profiles of AMPs in the Chinese shrimps, Fenneropenaeus chinensis, simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive, G + ) or Vibrio anguillarium (Gram-negative, G+). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium, and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore, the expression of these AMPs showed temporal expression profiles, suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.
关键词	antimicrobial peptides (AMPs) expression profiles bacteria Relish RNA interference
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China (Nos.31072203, 30230280) ; the Special Fund for Agro-Scientific Research in the Public Interest (No. 201103034) ; the National Basic Research Program of China (973 Program) (No. 2012CB114403)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/191186
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Wang DD,Li FH,Li SH,et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish. 2012.