Genetic transformation of marine cyanobacterium Synechococcus sp.CC9311(Cyanophyceae) by electroporation

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	Genetic transformation of marine cyanobacterium Synechococcus sp.CC9311(Cyanophyceae) by electroporation
	Chen HX(陈华新); Lin HZ(林瀚智); Jiang P(姜鹏); Li FC(李富超); Qin S(秦松)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2013-03-15
出版年	2013
卷号	v.31 期号:02 页码:172-176
文献类型	CNKI期刊论文
摘要	Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
关键词	chromatic acclimation electroporation genetic transformation Synechococcus sp.CC9311
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q943
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the Key Innovation Project of Institute of Oceanology,Chinese Academy of Sciences(No.2009-2) ; the Natural Science Foundation of Shandong Province(No.2009ZRB02542) ; the Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques,SOA(No.MBSMAT-2010-03) ; the National Natural Science Foundation of China(No.41276164) ; the Natural Science Foundation of Jiangsu Province(No.BK2012650)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190936
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences 3.YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Chen HX,Lin HZ,Jiang P,et al. Genetic transformation of marine cyanobacterium Synechococcus sp.CC9311(Cyanophyceae) by electroporation. 2013.